Refolding of Fully Reduced Bovine Pancreatic Trypsin(BIOCHEMICAL ENGINEERING)
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概要
- 論文の詳細を見る
Refolding of bovine pancreatic trypsin was carried out. When starting with denatured S-S intact trypsin, the recovered activity attained was 95-100%. In contrast, the recovered activity after refolding denatured S-S reduced trypsin was considerably low compared with other proteases that have been worked with previously. Such low recovered activity was attributed to the small amount of fully reduced trypsin used as starting material for complete refolding. Taking this into account, a recovered activity of 86% could be achieved when using inhibitor-immobilized gels.
- 社団法人日本生物工学会の論文
- 2008-10-25
著者
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Ohshima Yuji
Department Of Biomolecular Science Faculty Of Engineering Gifu University
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SUZUKI Yusuke
Department of Electrical Engineering and Computer Science, Nagoya University
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NOHARA DAISUKE
Department of Chemical Reaction Engineering, Faculty of Pharmaceutical Sciences, Nagoya City Univers
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Nakatani Akihiro
Department of Biomolecular Science, Faculty of Engineering, Gifu University
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Nakatani Akihiro
Department Of Biomolecular Science Faculty Of Engineering Gifu University
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Nakatani Akihiro
Department Of Adaptive Machine Systems Graduate School Of Engineering Osaka University
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Nohara Daisuke
Department Of Biomolecular Science Faculty Of Engineering Gifu University
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Suzuki Yusuke
Department Of Biomolecular Science Faculty Of Engineering Gifu University
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Suzuki Yusuke
Department Of Biological Production Akita Prefectural University
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