High Performance in Protease Refolding by Application of a Continuous System Using Immobilized Inhibitor(BIOCHEMICAL ENGINEERING)
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概要
- 論文の詳細を見る
In the reoxidative refolding of Streptomyces griseus trypsin, which is a serine protease having three S-S bonds per molecule, a synthetic inhibitor immobilized on agarose beads was applied in order to avoid the digestion of non-renatured protease molecules by renatured ones. A semi-continuous refolding system was fabricated by packing such inhibitor-immobilized gels in a glass column and the optimal operating conditions were investigated. Taking account of the effective conditions surveyed in the system, a continuous refolding system was constructed and operated to achieve higher performance. By application of the continuous system, a marked increase in the recovery rate as well as high recovered activity could be accomplished, i. e., the recovery rate obtained was ca. 40 times higher than that in the semi-continuous system. This system was also revealed to be substantially advantageous since it includes not only effective refolding but also separation, purification and enrichment processes in one operation.
- 社団法人日本生物工学会の論文
- 2004-12-25
著者
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KONDO Daisuke
Department of Medicine (II), Graduate School of Medical and Dental Sciences, Niigata University
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NOHARA DAISUKE
Department of Chemical Reaction Engineering, Faculty of Pharmaceutical Sciences, Nagoya City Univers
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KATOH Shigeo
Graduate School of Science and Technology, Kobe University
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Suzuki E
Hoechst Marion Roussel Ltd. Kawagoe Jpn
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Katoh Shigeo
Graduate School Of Science And Technology Kobe University
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Katoh Shigeo
Graduate School Of Engineering Department Of Chemical Science And Engineering Kobe University:depart
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Nohara Daisuke
Department Of Biomolecular Science Faculty Of Engineering Gifu University
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Nohara Daisuke
Department Of Biomolecular Science Faculty Of Engineering Gifu University:department Of Chemical Rea
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Suzuki Ei-ichiro
Institute Of Life Sciences Ajinomoto Co Inc
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鈴木 恵理香
Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Nagoya City University
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Kondo Daisuke
Department Of Chemical Reaction Engineering Nagoya City University
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