Elution Behavior of DNAs in Temperature-Gradient Affinity Chromatography for Single Nucleotide Polymorphism Detection
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概要
- 論文の詳細を見る
A single nucleotide polymorphism (SNP) was detected by the use of affinity chromatography with a single-stranded DNA ligand and temperature gradient elution. Because of the difference in thermostability between the ligand and sample single-stranded DNAs with and without SNPs, they were eluted as a peak with a shoulder by the temperature gradient elution. The sample DNAs with SNPs were detected as low as 0.1 pmol, and the elution behavior of DNAs with and without SNPs in this chromatography was predicted by a numerical calculation method based on a thermodynamic mass transfer model.
- 社団法人 化学工学会の論文
- 2006-10-01
著者
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KATOH Shigeo
Graduate School of Science and Technology, Kobe University
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Katsuda Tomohisa
Graduate School of Science and Technology, Kobe University
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Katsuda Tomohisa
Graduate School Of Science And Technology Kobe University
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Katoh Shigeo
Graduate School Of Engineering Department Of Chemical Science And Engineering Kobe University:depart
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Katoh Shigeo
Department Of Chemical Science And Engineering Graduate School Of Engineering Kobe University
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KAMURA Mitsumasa
Graduate School of Science and Technology, Kobe University
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NISHIWADA Yasushi
Graduate School of Science and Technology, Kobe University
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Nishiwada Yasushi
Graduate School Of Science And Technology Kobe University
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Kamura Mitsumasa
Graduate School Of Science And Technology Kobe University
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Katoh Shigeo
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University
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