A Rapid Method for Determination of the Association Constant of Monoclonal Anibodies

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概要

• 論文の詳細を見る

• A rapid ELISA method for measuring association constants of monoclonal antibodies against protein antigens is proposed. Two microtiter plates were used; one plate was used for equillibrating monoclonal antibody solutions of various concentrations with antigens in wells of the plate and the other for measuring the equilibrated concentrations of the antibody by ELISA. It was possible to determied the association constants within a few hours by this method and apply the method to association constants larger than those measured by the equilibrium dialysis method.

• 公益社団法人日本生物工学会の論文
• 1993-12-25

著者

• Terashima Masaaki

  Department Of Biosphere Sciences Kobe College

• Katoh S

  Graduate School Of Science And Technology Kobe University

• Katoh Shigeo

  Department Of Chemical Science And Engineering Graduate School Of Engineering Kobe University

• Terashima M

  Kyoto Univ. Kyoto Jpn

• Sada E

  Kyoto Univ. Kyoto

• Sada Eizo

  Department Of Chemical Engineering Faculty Of Engineering Kyoto Univeristy

• SOHMA YOSHINORI

  Department of Synthetic Chemistry and Biological Chemistry, Kyoto University

• TSUGAWA MAMORU

  Department of Synthetic Chemistry and Biological Chemistry, Kyoto University

• Tsugawa M

  Osaka Regional Taxation Brueau Osaka Jpn

• Sohma Yoshinori

  Department Of Synthetic Chemistry And Biological Chemistry Kyoto University

• KATOH SHIGEO

  Department of Chemical Engineering, Kyoto University

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