Rates of mass transfer in affinity chromatography.
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概要
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The volumetric coefficients of overall mass transfer in affinity chromatography were obtained for benzamidine, trypsin, and β-galactosidase in Sepharose 4B and 6B columns. The volumetric coefficients decreased with molecular weight and with the degree of cross-linking of the adsorbents. The column productivities of the Sepharose 6B-STI column were higher than those of the Sepharose 4B-STI column because of the high operating velocity. In affinity chromatography of β-galactosidase, on the other hand, the column productivity of the Sepharose 6B-PAPTG column was extremely lower than that of the Sepharose 4B-PAPTG column.
- 公益社団法人 化学工学会の論文
著者
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Sada Eizo
Department Of Chemical Engineering Faculty Of Engineering Kyoto Univeristy
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KATOH SHIGEO
Department of Chemical Engineering, Kyoto University
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