Purification of tyrosyl-tRNA synthetase by affinity chromatography.
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概要
- 論文の詳細を見る
Stable tyrosyl-tRNA synthetase (EC 6.1.1.1) was isolated from thermophilic bacterium Bacillus stearothermophilus and purified by use of two consecutive affinity chromatographic procedures on a large scale. The specific activity increased 25, 900-fold after the purification. With a Matrex Blue A column, used in the finalstep affinity Chromatography, the effects of factors influencing the performance of Chromatography were studied. The column diameter was scaled up from 1.65cm to 18.4cm without appreciable change in the elution profiles. The degree of purification decreased gradually and the elution profile became sharper with the increase in concentration of potassium chloride from 0.1 to 0.6 mol ⋅ 1-1. Variation of the elution profile with liquid velocity was also studied. The experimental elution curves showed general agreement with the calculated values based on the mass transfer theory.
- 公益社団法人 化学工学会の論文
著者
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Sada Eizo
Department Of Chemical Engineering Faculty Of Engineering Kyoto Univeristy
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INOUE TSUNEO
Department of Chemical Engineering, Kyoto University
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KATOH SHIGEO
Department of Chemical Engineering, Kyoto University
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MATSUKURA KAZUO
Research and Development Center, Unitika Ltd.
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SHIOZAWA MASAMI
Research and Development Center, Unitika Ltd.
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TAKEDA AKIRA
Research and Development Center, Unitika Ltd.
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