Epitope Screening by Use of a Random Peptide-Displayed Phage Library and Polyclonal Antibody-Coupled Liposomes

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概要

• 論文の詳細を見る

• Linear epitopes of sperm whale myoglobin for a rabbit polyclonal anti-myoglobin antibody were determined from a random-peptide displayed phage library by biopanning using antibody-coupled liposomes (AB-MLVs). Two kinds of positive phages displaying peptides (RNFGADAQ and YDLDIGAD) homological to the myoglobin sequence were obtained after 4 rounds of biopanning. Binding affinities of antibodies, which were fractionated from the anti-myoglobin antibody by specificity to both the peptides, were higher than that of an antibody fraction specific to the peptide that was screened by biopanning using an antibody-coated polystyrene tube (AB-Tube). The epitope identified in this study, N(D)FGADAQ has not been reported as an epitope of the rabbit anti-myoglobin antibody. These results suggest that biopanning using AB-MLVs shows much higher selectivity to recover phages displaying peptides with high affinity and specificity than that using the AB-Tube.

• 社団法人 化学工学会の論文
• 2006-11-01

著者

• Kumada Yoichi

  Department of Chemistry and Materials Technology, Kyoto Institute of Technology

• Kumada Yoichi

  Department Of Bioscience And Biotechnology Okayama University

• Terashima Masaaki

  Department Of Biosphere Sciences Kobe College

• Katoh Shigeo

  Graduate School Of Engineering Department Of Chemical Science And Engineering Kobe University:depart

• Katoh Shigeo

  Department Of Chemical Science And Engineering Graduate School Of Engineering Kobe University

• Nogami Masao

  Graduate School Of Science And Technology Kobe University

• HASAN Fida

  Graduate School of Science and Technology, Kobe University

• TERASHIMA Masaaki

  Graduate School of Human Sciences, Kobe College

• Katoh Shigeo

  Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University

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