N-linked glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells
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概要
- 論文の詳細を見る
The oligosaccharide structures of total glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells are determined. The N-linked sugar chains released by hydrazinolysis were labeled with 2-aminopyridine, and analyzed by a combination of HPLC, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and exoglucosidase digestion. The glycan structures determined were GlcNAcMan3FucXylGlcNAc2 (8.5%), Man3FucXylGlcNAc2 (70.0%), Man3XylGlcNAc2 (13.7%), and GlcNAcMan3XylGlcNAc2 (7.8%). All the glycan structures of glycoproteins from Arabidopsis MM2d cells used in this study contain typical plant bisecting either β1,2-xylose or α1,3-fucose residues, showing that suspension-cultured MM2d cells have a glycosylation potential similar to that of tobacco BY2 suspension-cultured cells.
- 日本植物細胞分子生物学会の論文
- 2007-03-01
著者
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Fujiyama Kazuhito
The International Center for Biotechnology, Osaka University
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SEKI Tatsuji
The International Center for Biotechnology, Osaka University
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Kazuhito Fujiyama
International Center For Biotechnology Osaka University
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Fujiyama Kazuhito
大阪大学生物工学国際交流センター
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Fujiyama Kazuhito
International Center For Biotechnology Osaka Univ.
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Fujiyama Kazuhito
Department Of Fermentation Technology Osaka University
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Fujiyama Kazuhito
International Center For Biotechnology Osaka University
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Seki Tatsuji
The International Center For Biotechnology Osaka University
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Tanaka Ryoji
The International Center for Biotechnology, Osaka University
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TERASHIMA Masaaki
Department of Biosphere Sciences, School of Human Sciences, Kobe College
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Fujiyama Kazuhito
The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita-shi Osaka 565-0871 Jpn
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Terashima Masaaki
Department Of Biosphere Sciences Kobe College
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Terashima Masaaki
Department Of Biosphere Sciences School Of Human Sciences Kobe College
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Tanaka Ryoji
The International Center For Biotechnology Osaka University
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Fujiyama Kazuhito
The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita Osaka 565-0871 Jpn
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