Characteristic of the Oligosaccharide Structure of Jack Bean α-Mannosidase with Some Lectins
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概要
- 論文の詳細を見る
An α-mannosidase was purified over 90-fold from jack bean seedlings by succesive chromatography with an overall yield of 6%. Electrophoresis under denaturing conditions gave two subunits with molecular masses of 66,000 (H-subunit) and 44,000 (L-subunit), respectively. With periodic acid-Schiff reagent, only the H-subunit was stained for carbohydrate. Upon N-Glycanase digestion, the molecular mass of the H-subunit was slightly reduced. Binding experiments of the protein toward some lectins suggested that the subunit of the α-mannosidase contained not an O-linked, but an N-linked oligosaccharide, which is either a high mannose or complex type, without a nonreducing terminal mannose or a sialic acid residue.
- 社団法人日本生物工学会の論文
- 1994-09-25
著者
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Fujiyama Kazuhito
International Center for Biotechnology, Osaka University
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YOSHIDA Toshiomi
International Center for Biotechnology, Osaka University
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Fujiyama Kazuhito
International Center For Biotechnology Osaka Univ.
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Yoshida Toshiomi
International Center For Biotechnology Osaka University
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Oh G‐s
Osaka Univ. Osaka Jpn
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Oh Gyu-seop
International Center For Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universi
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ARAKI KEITA
International Center of Cooperative Research in Biotechnology, Japan (ICBiotech), Faculty of Enginee
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Araki Keita
International Center Of Cooperative Research In Biotechnology Japan (icbiotech) Faculty Of Engineeri
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Fujiyama Kazuhito
International Center Of Cooperative Research In Biotechnology Japan (icbiotech) Faculty Of Engineeri
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Yoshida Toshiomi
International Center Of Cooperative Research In Biotechnology Japan (icbiotech) Faculty Of Engineeri
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Yoshida Toshiomi
Internatioal Center For Biotechnology Osaka University
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