Transduction of Static Pressure Signal to Expression of Human Granurocytes Macrophage Colony Stimulating Factor Mrna in Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)

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概要

• 論文の詳細を見る

• The expression level of human granurocytes macrophage colony stimulating factor (hGM-CSF) mRNA in Chinese hamster ovary (CHO) DR1000L4N cells increased by 24% with pressurization. Treatment of cells with chelerythrine chloride (10nM, 15min), an inhibitor of protein kinase C (PKC), did not suppress the pressure-induced (0.9MPa) expression of hGM-CSF mRNA, while it decreased the hGM-CSF gene expression level at normal pressure (0.1MPa). Treatment with U0126 (20μM, 60min), a specific inhibitor of extracellular signal-related kinase (ERK1/2), decreased the expression level of hGM-CSF mRNA at 0.1MPa to 80% of that without U0126 treatment. Similarly, treatment with U0126 decerased the pressure-induced (0.9MPa) expression level of hGM-CSF mRNA to 79% of the control expression level at 0.1Mpa without treatment. The amount of intracellular phosphorylated ERK1/2 increased with pressurization (0.9MPa). These results suggest that the pressure-induced expression of hGM-CSF mRNA in CHO DR1000L4N cells depends not on PKC but the ERK1/2 signaling cascade.

• 社団法人日本生物工学会の論文
• 2003-07-25

著者

• TAKAGI Mutsumi

  International Center for Biotechnology, Osaka University

• YOSHIDA Toshiomi

  International Center for Biotechnology, Osaka University

• Yoshida Toshiomi

  大阪大学生物工学国際交流センター

• Yoshida Toshiomi

  International Center Of Cooperative Research In Biotechnology Faculty Of Engineering Osaka Universit

• Yoshida T

  International Center For Biotechnology Osaka University

• Yoshida Toshiomi

  International Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大・工・生命工

• Takagi Mutsumi

  International Center For Biotechnology Osaka University:(present Address)division Of Biotechnology A

• Takagi M

  International Center For Biotechnology Osaka University

• Takagi M

  Japan Advanced Inst. Of Sci. And Technol. Ishikawa Jpn

• Takagi M

  Osaka Univ. Osaka Jpn

• GONG HENG

  International Center for Biotechnology, Osaka University

• Gong Heng

  International Center For Biotechnology Osaka University

• Yoshida T

  Division Of Material And Biological Graduate School Japan Women's University

• Yoshida T

  Kinki Univ. Nara Jpn

• Yoshida Toyokazu

  Dept. Food Nutri. Fac. Agr. Kinki Univ.

• Takagi Mutsumi

  Internatioal Center For Biotechnology Osaka University

• Takagi Mutsumi

  School Of Materials Science Japan Advanced Institute Of Science And Technology

• Yoshida T

  Department Of Biomolecular Science Gifu University

• Yoshida Toshiomi

  Internatioal Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大 工

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