Role of the Pre-Pro-Region of Neutral Protease in Secretion in Bacillus subtilis
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概要
- 論文の詳細を見る
A thernistable neutral protease (NprT) form Bacillus stearothemophilus CU21 is translated as a precursor containing pre-pro structure. Deletion and insertion mutations were introduced in the pro-region and the effects of mutations on production of active NprT were studied. The pro-region was important for the production of active NprT and was effective only when it was combined with the signal sequence(pre-region) of NprT. To examine the role of the pre-region, a penicillinase gene from Bacillus licheniformis (penP) was fused with various nprT secretion vectors. In was also found that the pre-region of NprT can potentially function as a signal sequence but is more functional for the production of NprT when it was combined with a normal pro-region. We discuss how the processing between the pro-structure and the mature portion of the protease might be done autoproteolytically.
- 社団法人日本生物工学会の論文
- 1989-02-25
著者
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TAKAGI Mutsumi
International Center for Biotechnology, Osaka University
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IMANAKA Tadayuki
Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University
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TAKAGI MASAHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Takagi M
Asahi Chemical Industry Co. Ltd. Shizuoka Jpn
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Maeda Takuya
Department Of Biotechnology Faculty Of Eigineering Osaka University
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Imanaka Tadayuki
Department Of Applied Biotechnology Faculty Of Engineering Osaka University
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Takagi M
Japan Advanced Inst. Sci. And Technol. Ishikawa Jpn
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Takagi Masahiro
Department Of Biotechnology Faculty Of Engineering Osaka University
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