Cloning and Nucleotide Sequence of the Regulatory Gene, degM, for Minor Serine Protease in Baciilus subtilis
スポンサーリンク
概要
- 論文の詳細を見る
The regulatory gene (degM) from Bacillus sp. B21-2 which enhanced the production of minor serine protease was cloned in Bacillus subtilis DB104 (Npr^- Apr^-). Nucleotide sequence analysis showed that the degM gene was composed of 1119 bases (373 amino acid residues). The deduced amino acid sequence was fairly homologous with those of sensor proteins of a two-component regulatory system in Bacillus sp.
- 社団法人日本生物工学会の論文
- 1992-10-25
著者
-
TAKAGI Mutsumi
International Center for Biotechnology, Osaka University
-
IMANAKA Tadayuki
Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University
-
TAKAGI MASAHIRO
Department of Biotechnology, Graduate School of Engineering, Osaka University
-
Takagi M
Asahi Chemical Industry Co. Ltd. Shizuoka Jpn
-
Maeda Takuya
Department Of Biotechnology Faculty Of Eigineering Osaka University
-
Imanaka Tadayuki
Department Of Applied Biotechnology Faculty Of Engineering Osaka University
-
Takagi M
Japan Advanced Inst. Sci. And Technol. Ishikawa Jpn
-
MASUI AKIHIKO
Technology Research Institute of Osaka Prefecture
-
FUJIWARA NOBUAKI
Technology Research Institute of Osaka Prefecture
-
MASUI AKIHIKO
Osaka Prefectural Industrial Technology Research Institute
-
FUJIWARA NOBUAKI
Osaka Prefectural Industrial Technology Research Institute
-
Fujiwara N
Technology Research Institute Of Osaka Prefecture
-
Fujiwara Nobuaki
Osaka Prefectural Industrial Research Institute
-
Takagi Masahiro
Department Of Biotechnology Faculty Of Engineering Osaka University
関連論文
- Effect of heparin addition on expansion of cord blood hematopoietic progenitor cells in three-dimensional coculture with stromal cells in nonwoven fabrics
- Analysis of Hematopoietic Microenvironment Containing Spatial Development of Stromal Cells in Nonwoven Fabrics (ANIMAL BIOTECHNOLOGY)
- Control of Cell Growth Rate by Sugar Feeding Based on CO_2 Production Rate
- Production of S-(+)-Ibuprofen with High Optical Purity from a Nitrile Compound by Cells immobilized on Cellulose Porous Beads
- Production of S-(+)-Ibuprofen from a Nitrile Compound by Enzymatic Reaction Combined with Ultrafiltration
- Transduction of Static Pressure Signal to Expression of Human Granurocytes Macrophage Colony Stimulating Factor Mrna in Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
- Effect of Static Pressure on Human Granulocyte-Macrophage Stimulating Factor (hGM-CSF) Production by Chinese Hamster Ovary Cells
- Effects of Shifts Up and Down in Osmotic Pressure on Production of Tissue Plasminogen Activator by Chinese Hamster Ovary Cells in Suspension
- Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes
- Effect of Poly DL-Lactic-Co-Glycolic Acid Mesh on a Three-Dimensional Culture of Chondrocytes(CELL AND TISSUE ENGINEERING)
- Bacterial Alginate Lyase : Enzymology, Genetics and Application
- Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells^1
- Utilization of Immobilized Archaeal Chaperonin for Enzyme Stabilization
- Enhanced Signal Transduction by a Directly Fused Protein of Interleukin-6 and Its Receptor
- Unique Nucleoid Structure during Cell Division of Thermococcus kodakaraensis KOD1
- Alteration of Product Specificity of Cyclodextrin Glucanotransferase from Thermococcus sp.B1001 by Site-Directed Mutagenesis
- Effect of Polyamines on Histone-Induced DNA Compaction of Hyperthermophilic Archaea
- Highly Branched Oligosaccharides Produced by the Transglycosylation Reaction of Neopullulanase
- Hydrodynamically stable adhesion of endothelial cells onto a polypropylene hollow fiber membrane by modification with adhesive protein
- Enhanced adhesion of endothelial cells onto a polypropylene hellow-fiber membrane by plasma discharge treatment and high inoculum cell density
- Gene Cloning and Sequence Analysis of Cobyric Acid Synthase and Cobalamin (5'-phosphate) Synthase from Hyperthermophilic Archaeon Pyrococcus sp. KOD1
- Biophysical Analysis of Heat-Induced Structural Maturation of Glutamate Dehydrogenase from a Hyperthermophilic Archaeon (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Mutational Effects on O^6-Methylguanine-DNA Methyltransferase from Hyperthermophile : Contribution of Ion-Pair Network to Protein Thermostability
- Contribution of Protein-Surface Ion Pairs of a Hyperthermophilic Protein on Thermal and Thermodynamic Stability
- Genetic, Enzymatic, and Structural Analyses of Phenylalanyl-tRNA Synthetase from Thermococcus kodakaraensis KOD1
- Biophysical Effect of Amino Acids on the Prevention of Protein Aggregation
- 超好熱菌由来タンパク質のさまざまな変性条件下での構造安定性
- Acceptor Specificity of 4-α-Glucanotransferase from Pyrococcus kodakaraensis KOD1, and Synthesis of Cycloamylose
- Characterization of FtsZ Homolog from Hyperthermophilic Archaeon Pyrococcus kodakaraensis KOD1
- Characterization and Application to Hot Start PCR of Neutralizing Monoclonal Antibodies against KOD DNA Polymerase
- Gene Analysis and Enzymatic Properties of Thermostable β-Glycosidase from Pyrococcus kodakaraensis KODI
- Crystallographic Studies on a Family B DNA Polymerase from Hyperthermophilic Archaeon Pyrococcus kodakaraensis Strain KOD1^1
- Phylogenetic Analysis and Effect of Heat on Conformational Change of Ferredoxin from Hyperthermophilic Archaeon Pyrococcus sp.KOD1
- Cloning and Expression of the 4-α-Glucanotransferase Gene from the Hyperthermophilic Archaeon Pyrococcus sp. KOD1,and Characterization of the Enzyme
- Cloning and Expression of the α-Amylase Gene from the Hyperthermophilic Archaeon Pyrococcus sp. KOD1,and Characterization of the Enzyme
- Vector Mosquitoes of Japanese Encephalitis (Diptera: Culicidae) in Northern Thailand: Seasonal Changes in Larval Community Structure
- SEASONAL AND SPATIAL DISTRIBUTION OF 3 MALARIA VECTORS AT THREE MOUNTAINOUS VILLAGES IN NORTH THAILAND
- SEASONAL DENSITY AND MALARIA VECTOR COMPETENCE OF ANOPHELES MINIMUS AND OTHER ANOPHELINES AT A SHALLOW VALLEY IN NORTH THAILAND
- Evaluation of effective period of a juvenile hormone mimic, pyriproxyfen, against Aedes albopictus : Preliminary experiments in the laboratory and the field.
- Production of Bacterial Alginate-Specific Lyase by Recombinant Bacillus subtilis
- Crystallization and Preliminary X-Ray Diffraction Studies of Bacterial Alginate Lyase
- Metabolic Flux Change in Hybridoma Cells under High Osmotic Pressure
- 524 Improvement of Monoclonal Antibody Production with Gradual Increase of Osmotic Pressure
- Effect of Insoluble Medium Component on the Productivity of Tissue Plasminogen Activator by Cells on Microcarriers
- An Oxygen Supply Strategy for the Large-Scale Production of Tissue Plasminogen Activator by Microcarrier Cell Culture
- Gene Cloning and Characterization of Aldehyde Dehydrogenase from a Petroleum-Degrading Bacterium, Strain HD-1
- Effect of Sugar Residues in a Glycolipid Coated onto a Dish on Ammonia Consumption and Gluconeogenesis Activity of Primary Rat Hepatocytes
- Synthesis and Evaluation of Novel Peptide-Lipid
- Effect of galactose residue in glycolipid coated onto a dish on ammonia consumption activity of primary rat hepatocytes
- Expanded Bed Adsorption for Purification of Alcohol Dehydrogenase Using a Dye-Iminodiacetic Acid Matrix (BIOCHEMICAL ENGINEERING)
- Multivalent Binding Interaction of Alcohol Dehydrogenase on Dye-Metal Affinity Matrix(BIOCHEMICAL ENGINEERING)
- Development of New Dye-Metal Agarose-Coated Alumina Matrix and Elution Strategy for Purification of Alcohol Dehydrogenase
- 336 Development of polyethyleneimine-coated alumina for dye-affinity chromatography
- Characterization of Petroleum-Degrading Bacteria from Oil-Contaminated Sites in Vietnam
- Equilibrium and Kinetic Stability of a Hyperthermophilic Protein, O^6-Methylguanine-DNA Methyltransferase under Various Extreme Conditions
- Rapid Method for Detection and Detoxification of Heavy Metal Ions in Water Environments Using Phytochelatin
- A Simple Method for Determination of Substrate Specificity of Alginate Lyases
- Purification and Characterization of α-Glucan Phosphorylase from Bacillus stearothermophilus
- Production and Some Properties of a Dextrin with a Narrow Size Distribution by the Cyclization Reaction of Branching Enzyme
- Expression of Recombinant Antibody against Cancer-Specific Carbohydrate
- COMPLETE GENOME ANALYSIS AND TARGETED GENE DISRUPTION IN THE HYPERTHERMOPHILIC ARCHAEON, THERMOCOCCUS KODAKARAENSIS(Life under Extreme Environments, International Symposium on Origins of Life and Astrobiology)
- Gene Cloning and Characterization of Fructose-1,6-Bisphosphate Aldolase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1
- Repeated Addition of Insulin for Dynamic Control of Apoptosis in Serum-Free Culture of Chinese Hamster Ovary Cells (ANIMAL BIOTECHNOLOGY)
- Combined Addition of Glutathione and Iron Chelators for Decrease of Intracellular Level of Reactive Oxygen Species and Death of Chinese Hamster Ovary Cells
- Effect of Antioxidants on the Apoptosis of CHO Cells and Production of Tissue Plasminogen Activator in Suspension Culture
- Display of Heterologous Gene Products on the Escherichia coli Cell Surface as Fusion Proteins with Flagellin
- Gentisate 1,2-dioxygenase from Xanthobacter polyaromaticivorans 127W.
- Isolation and Characterization of Xanthobacter polyaromaticivorans sp. nov. 127W That Degrades Polycyclic and Heterocyclic Aromatic Compounds under Extremely Low Oxygen Conditions
- In Vitro Proliferation of Primary Rat Hepatocytes Expressing Ureogenesis Activity by Coculture with STO Cells
- Effects of High Concentrations of Energy Sources and Metabolites on Suspension Culture of Chinese Hamster Ovary Cells Producing Tissue Plasminogen Activator
- Effect of Mixing Time on Taxoid Production Using Suspension Cultures of Taxus chinensis in a Centrifugal Impeller Bioreactor
- Effect of pore diameter of a porous membrane on progenitor cell content during membrane-separated coculture of hematopoietic cells and a stromal cell line
- Starch Particles Modified with Gelatin as Novel Small Carriers for Mammalian Cells
- Nitrogen depletion for intracellular triglyceride accumulation to enhance liquefaction yield of marine microalgal cells into a fuel oil
- Effect of Hydrostatic Pressure on Hybridoma Cell Metabolism
- Effect of Temperature on Cell Population Balance in Dexter's Culture of Murine Bone Marrow Hematopoietic Cells with Stromal Cells
- Selective Retension of Active Cells Employing Low Centrifugal Force at the Medium Change during Suspension Culture of Chinese Hamster Ovary Cells Producing tPA
- Acetone-Butanol Fermentation by Clostridium aurantibutyricum ATCC 17777 from a Model Medium for Palm Oil Mill Efflluent
- Characterization of a Small Metalloprotease from Streptomyces caespitosus with High Specificity to Aromatic Residues
- Production and Characterization of Biosurfactants from Bacillus licheniformis F2.2(Microbiology & Fermentation Technology)
- Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells.
- Effect of Concentrations of Murine Stromal and Hematopoietic Cells on the Progenitors Expansion in Their Three-Dimensional Coculture in Nonwoven Fabrics
- Highly Thermostable Neutral Protease from Bacillus stearothemophilus
- Effect of Growth Temperature and Growth Phase on the Lipid Composition of the Archaeal Membrane from Thermococcus kodakaraensis
- Diversity in Size and Alkaliphily of Thermostable α-Amylase-Pullulanases (AapT) Produced by Recombinant Escherichia coli, Bacillus subtilis and the Wild-Type Bacillus sp.
- Rational Design for Stabilization and Optimum pH Shift of Serine Protease AprN
- Nucleotide Sequence and Possible Functional Mechanism of the Transcriptional Activator Gene (preL) for Neutral Protease from Lactobacillus sp.
- Nucleotide Sequence of the Neutral Protease Gene (nprL) from Lactobacillus sp. and Characterization of the Enzyme
- Purification and Characterization of a New Metal Protease Which Hydrolyzes the Cyclic Decapeptide, Gramicidin S
- Cloning and Nucleotide Sequence of the Regulatory Gene, degM, for Minor Serine Protease in Baciilus subtilis
- Role of the Pre-Pro-Region of Neutral Protease in Secretion in Bacillus subtilis
- Isolation and Characterization of Psychrotrophs from Subterranean Environments
- High-Level Production of Erythritol by Strain 618A-01 Isolated from Pollen
- Isolation of Bacterial Strans Colonizable in Mosquito Larval Guts as Novel Host Cells for Mosquito Control
- Identification of the Gene Encoding Esterase, a Homolog of Hormone-Sensitive Lipase, from an Oil-Degrading Bacterium, Strain HD-1
- Gentisate 1,2-Dioxygenase from Xanthobacter polyaromaticivorans 127W
- The Concept of the α-Amylase Family : Structural Similarity and Common Catalytic Mechanism
- Cloning and Expression of the BamHI Restriction-Modification System in Bacillus subtilis
- Effect of Growth Temperature and Growth Phase on the Lipid Composition of the Archaeal Membrane from Thermococcus kodakaraensis
- Identification of the Phr-dependent heat shock regulon in the hyperthermophilic archaeon, Thermococcus kodakaraensis