On-Line Monitoring and Control of Aceotone-Butanol Fermentation by Membrane-Sensor Mass Spectrometry

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概要

• 論文の詳細を見る

• A mass spectrometry (MS) membrane sensor was developed and applied to on-line product measurement in acetone-butanol fermentation. The sensor facillitated the monitoring of acetone, butanol. ethanol. H_2 and CO_2 and single-compound calibration curves for both acetone and butanol showed a linear relationship between the product concentration and the MS response. However, when an actual fermentation was monitored, the product concentration calculated from the MS response wqs smaller than the concentration determined by gas chromatography, and the relationshiop between the response and the product concentration was nonlinear. It was found that large amounts of geses (H_2,CO_2) entering the MS analyzation chamber were causing a 'space charge effect', which resulted in an MS response celling. The problem could be resolved by reducing the surface area of the sensor membrane. Under some fermentation conditions, a by-product, n-butyl butyrate, was produced, and this interfered with the measurement of butanol due to a peak overlapping effect. However, it was found that this could be compensated for by using an empirical equation. Application of the MS membrane sensor in a fed batch culture of acetone-butanol fermentation resulted in succesful control of the buthanol concentration.

• 社団法人日本生物工学会の論文
• 1995-03-25

著者

• Fujiyama Kazuhito

  International Center for Biotechnology, Osaka University

• YOSHIDA Toshiomi

  International Center for Biotechnology, Osaka University

• SEKI Tatsuji

  International Center for Biotechnology, Osaka University

• Kazuhito Fujiyama

  International Center For Biotechnology Osaka University

• Fujiyama Kazuhito

  大阪大学生物工学国際交流センター

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka Univ.

• Fujiyama Kazuhito

  Department Of Fermentation Technology Osaka University

• Fujiyama Kazuhito

  International Center For Biotechnology Osaka University

• Seki Tatsuji

  International Center For Biotechnology Osaka University

• Seki Tatsuji

  大阪大学生物工学国際交流センター

• Yoshida Toshiomi

  The International Center For Biotechnology Osaka University

• Yoshida Toshiomi

  International Center For Biotechnology Osaka University

• 吉田 豊和

  岐阜大・工・生命工

• CHAUVATCHARIN SOMCHAI

  International Center of Cooperative Research in Biotechnology, Osaka University

• Chauvatcharin Somchai

  International Center Of Cooperative Research In Biotechnology Osaka University

• Yoshida Toshiomi

  Internatioal Center For Biotechnology Osaka University

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