Change in glycosylation pattern with extension of endoplasmic reticulum retention signal sequence of mouse antibody produced by suspension-cultured tobacco BY2 cells(PLANT BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
The production of antibodies using plants as bioreactors has been realized. Because sugar chain structures on recombinant proteins are a cause of concern, remodeling technology is highly promising. Localizing recombinant proteins in the endoplasmic reticulum (ER) affects the glycosylation pattern in transgenic plants. Previously, a mouse antibody produced by suspension-cultured tobacco BY2 cells has sugar chains with possible glycoepitopes as the predominant structures. In this study, we extended the Lys-Asp-Glu-Leu (KDEL) ER retention signal sequence over the heavy (H) and light (L) chains of the antibody and expressed the altered antibody in tobacco BY2 cells to study the effect of the KDEL sequence on glycosylation. For the antibody with the KDEL-extended H-chains, glycans with β(1,2)-xylose or α(1,3)-fucose residues accounted for 49% of the total glycans. Meanwhile, for the antibody with the KDEL-extended H- and L-chains, glycans with xylose or fucose accounted for 38% of the total glycans. Although the addition of an ER retention signal shifted the dominant glycan structures of the KDEL-extended antibody to high-mannose-type structures, some of the antibodies escaped the retrieval system during intracellular traffic and were then modified by xylosylation or fucosylation.
- 社団法人日本生物工学会の論文
著者
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Fujiyama Kazuhito
International Center for Biotechnology, Osaka University
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Omasa Takeshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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大政 健史
大阪大学大学院工学研究科
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Omasa Takeshi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Omasa Takeshi
大阪大学生物工学国際交流センター
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SAKAI Yohei
International Center for Biotechnology, Osaka University
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MISAKI Ryo
International Center for Biotechnology, Osaka University
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SEKI Tatsuji
International Center for Biotechnology, Osaka University
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Kazuhito Fujiyama
International Center For Biotechnology Osaka University
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Fujiyama Kazuhito
大阪大学生物工学国際交流センター
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Fujiyama Kazuhito
International Center For Biotechnology Osaka Univ.
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Fujiyama Kazuhito
Department Of Fermentation Technology Osaka University
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Fujiyama Kazuhito
International Center For Biotechnology Osaka University
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Misaki Ryo
International Center For Biotechnology Osaka University
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Misaki Ryo
The International Center For Biotechnology Osaka University
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Seki Tatsuji
International Center For Biotechnology Osaka University
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Seki Tatsuji
大阪大学生物工学国際交流センター
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Sakai Yohei
International Center For Biotechnology Osaka University
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Fujiyama Kazuhito
The International Center For Biotechnology Osaka Univ. 2-1 Yamada-oka Suita Osaka 565-0871 Jpn
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Omasa Takeshi
Department Of Biological Science And Technology Institute Of Technology And Science The University O
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Omasa Takeshi
Department Of Biological Science And Technology Biosystems Engineering Institute Of Technology And Science The University Of Tokushima
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