Decrease in Antithrombin III Fucosylation by Expressing GDP-fucose Transporter siRNA in Chinese Hamster Ovary Cells(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
The full-length cDNA of GDP-fucose transporter (GFT) was cloned from Chinese hamster ovary (CHO) 13D-35D cells. The predicted product consisted of 366 amino acids with 86% and 92% similarities to human GFT and mouse GFT, respectively. When synthetic small interfering RNA (siRNA) against CHO GFT was introduced into human antithrombin III (AT-III)-producing recombinant CHO cells, defucosylated AT-III increased by 30-40% after 3-d incubation. A GFT siRNA expression plasmid was also constructed to defucosylate recombinant AT-III. CHO cells, which were transfected with the siRNA expression plasmid, showed approximately 75% reduction of Golgi-GDP fucose, resulting in 10-40% increase in defucosylated AT-III.
- 社団法人日本生物工学会の論文
- 2008-08-25
著者
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大政 健史
大阪大学大学院工学研究科生命先端工学専攻
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Honda Kohsuke
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Omasa Takeshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Ohtake Hisao
Department of Biotechnology, Graduate School of Engineering, Osaka University
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岸本 通雅
京都工芸繊維大学工芸科学研究科
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Kishimoto Michimasa
Department of Chemistry and Materials Technology, Kyoto Institute of Technology
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Tanaka Ryo
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Doi Tomohiro
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Ando Masayuki
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kitamoto Yuka
Department of Biotechnology, Graduate School of Engineering, Osaka University
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大政 健史
大阪大学大学院工学研究科
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Omasa Takeshi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Omasa Takeshi
大阪大学生物工学国際交流センター
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Ohtake H
Department Of Biotechnology Osaka University
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Ohtake Hisao
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ohtake H
Institute Of Applied Microbiology University Of Tokyo
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Ohtake Hisao
Institute Of Applied Microbiology The University Of Tokyo
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Honda Kohsuke
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Honda Kohsuke
Grad. Sch. Of Eng. Osaka Univ.
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Honda Kohsuke
大阪大学 工学研究科生命先端工学専攻
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Ohtake Hisao
Department Of Fermentation Technology Hiroshima University
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Doi Tomohiro
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kitamoto Yuka
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Okumura H
Electrotechnical Lab. Ibaraki Jpn
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Kishimoto Michimasa
Department Of Chemistry And Materials Technology Kyoto Institute Of Technology
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Kishimoto Michimasa
Deparmtnet Of Biotechnology Osaka University
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Ohtake Hisao
大阪大学 工学研究科生命先端工学専攻
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Ando Masayuki
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Tanaka Ryo
Department Of Anesthesiology Kitasato University School Of Medicine
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Tanaka Ryo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Omasa Takeshi
Department Of Biological Science And Technology Institute Of Technology And Science The University O
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岸本 通雅
京工繊院・工芸・生体分子工
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岸本 通雅
京都工芸繊維大学大学院工芸科学研究科
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Omasa Takeshi
Department Of Biological Science And Technology Biosystems Engineering Institute Of Technology And Science The University Of Tokushima
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岸本 通雅
京工繊大院・工芸科学・生体分子工
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Okumura Hajime
Department of Biotechnology, Graduate School of Engineering, Osaka University
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