Isolation and Characterization of cbbL and cbbS Genes Encoding Form I Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large and Small Subunits in Nitrosomonas sp. Strain ENI-11(Microbiology & Fermentation Technology)

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概要

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• The cbbL and cbbS genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large and small subunits in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11 were cloned and sequenced. The deduced gene products, CbbL and CbbS, had 93 and 87% identity with Thiobacillus intermedius CbbL and Nitrobacter winogradskyi CbbS, respectively. Expression of cbbL and cbbS in Escherichia coli led to the detection of RubisCO activity in the presence of 0.1 mM isopropyl-β-D-thiogalactopyranoside (IPTG). To our knowledge, this is the first paper to report the genes involved in the carbon fixation reaction in chemolithotrophic ammonia-oxidizing bacteria.

• 社団法人日本農芸化学会の論文
• 2002-03-23

著者

• Ohtake Hisao

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• Kato Junichi

  Department Of Cardiology Asahikawa Medical College

• KURODA Akio

  Department of Molecular Biotechnology, Hiroshima University

• Ohtake H

  Department Of Biotechnology Osaka University

• Ohtake Hisao

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Ohtake H

  Institute Of Applied Microbiology University Of Tokyo

• Ohtake Hisao

  Institute Of Applied Microbiology The University Of Tokyo

• Ohtake Hisao

  Department Of Fermentation Technology Hiroshima University

• Hirota Ryuichi

  Department of Molecular Biotechnology, Hiroshima University

• Kato J

  Department Of Molecular Biotechnology Hiroshima University

• IKEDA TSUKASA

  Department of Fermentation Technology, Hiroshima University

• TAKIGUCHI NOBORU

  Department of Fermentation Technology, Hiroshima University

• MORITA Hiromu

  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni

• Kuroda A

  Department Of Molecular Biotechnology Hiroshima University

• Kuroda Akio

  Department Of Molecular Biotechnology Hiroshima University

• Kato Junichi

  Department Of Agricultural Biological Chemistry College Of Bioresource Sciences Nihon University

• Kato J

  Department Of Chemical Engineering Faculty Of Engineering Shizuoka University

• Morita Hiromu

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive

• Ikeda Tsukasa

  Department Of Applied Chemistry Utsunomiya University

• Ikeda T

  Dep. Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univ.

• Takiguchi Noboru

  Department Of Molecular Biotechnology Hiroshima University

• Takiguchi Noboru

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Kato Junichi

  Department Of Agricultural Chemistry University Of Tokyo

• Hirota Ryuichi

  Department Of Molecular Biotechnology Hiroshima University

• Ikeda T

  Osaka Medical Coll. Osaka

• Kuroda Akio

  Department Of Chemistry Faculty Of Science Nagoya University

• Hirota Ryuichi

  Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima University

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