The aarC Gene Responsible for Acetic Acid Assimilation Confers Acetic Acid Resistance on Acetobacter aceti

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概要

• 論文の詳細を見る

• Acetic acid resistance, which characterizes the genus Acetobacter, is conferred by three genes designated aarA, aarB, and aarC. The nucleotide sequence of aarC revealed that it coded for a protein of 492 amino acid residues. This gene product showed significant homology, with 44% identity, with the Neurospora crassa acu-8 gene product which is involved in acetate-utilization in this fungus. Consistent with this, and aarC-defective mutant, which was constructed by gene disruption with the cloned gene, showed an inability to assimilate acetic acid. Introduction of the aarC gene into the mutant restored both the resistance to acetic acid and the ability to assimilate acetic acid. These findings suggested that the aarC gene product was responsible for acetate assimilation and that the acetate-sensitive phenotype of the aarC mutant was caused by a defect in acetate assimilation.

• 社団法人日本生物工学会の論文
• 1993-10-25

著者

• Ohtake H

  Institute Of Applied Microbiology University Of Tokyo

• Ohtake Hisao

  Institute Of Applied Microbiology The University Of Tokyo

• Ohtake Hisao

  Department Of Fermentation Technology Hiroshima University

• FUKAYA MASAHIRO

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.,

• OKUMURA HAJIME

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.,

• KAWAMURA YOSHIYA

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.,

• HORINOUCHI Sueharu

  Department of Biotechnology, University of Tokyo

• Fukuwaka Masa-aki

  Hokkaido National Fisheries Research Institute

• Toda K

  Univ. Tokyo Tokyo Jpn

• Toda K

  Department Of Applied Chemistry And Biotechnology Niigata Institute Of Technology

• Toda K

  Faculty Of Engineering Niigata Institute Of Technology

• BEPPU Teruhiko

  Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok

• Tayama Kenji

  Nakano Biochemical Research Institute Nakano Vinegar Co. Ltd.

• Takemura Hiroshi

  (present Address)bio-polymer Research Co. Ltd.

• Takemura Hiroshi

  Bio-polymer Research Co. Ltd.:(present Address)nakano Central Institute Nakano Vinegar Co. Ltd.

• Horinouchi Sueharu

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Horinouchi Sueharu

  Dep. Of Biotechnology Graduate School Of Agriculture And Life Sciences Univ. Of Tokyo

• Beppu T

  Department Of Applied Biological Science Nihon University

• Toda Kiyoshi

  Faculty Of Engineering Niigata Institute Of Technology

• Kawamura Y

  National Food Research Inst. Tsukuba Jpn

• TAKEMURA HIROSHI

  Nakano Central Research Institute, Nakano Vinegar Co., Ltd.,

• Kawamura Y

  Tsuruga Institute Of Biotecnology Tokyo Co. Ltd.

• Fukaya M

  Central Research Institute Mitsukan Group Corporation

• Tagami Haruko

  Nakano Central Research Institute Nakano Vinegar Co. Ltd.

• Beppu Teruhiko

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• FUKAYA Masahiro

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.

• OKUMURA Hajime

  Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.

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