Improved Transformation Method for Acetobacter with Plasmid DNA
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概要
- 論文の詳細を見る
An improved method for transformation of derivative strains of A. aceti subsp. aceti No. 1023 with plasmid DNA has been developed. Addition of polyethylene glycol or dimethylsulfoxide increased the transformation efficiency by a factor of about ten. In the presence of PEG 4, 000, various transformation conditions were examined. Cells were also made transformation competent by treatment with other divalent cations than Ca2+. The pH of the buffer did not affect the efficiency significantly. The growth phase influenced the efficiency. Mutants showing high competence were derived by treatment with N-methyl-N-nitro-N-nitrosoguanidine. By the improved method using the highly transformable mutants, a transformation efficiency of approximately 105 transformants per μg plasmid DNA was achieved.
- 社団法人 日本農芸化学会の論文
著者
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OKUMURA HAJIME
Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.,
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Tayama Kenji
Nakano Biochemical Research Institute Nakano Vinegar Co. Ltd.
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Masai Hiroshi
Nakano Biochemical Research Institute Nakano Vinegar Co. Ltd.
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Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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FUKAYA Masahiro
Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.
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OKUMURA Hajime
Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd.
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