Production of Chymosin in Escherichia coli Cells and Its Enzymatic Properties
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概要
- 論文の詳細を見る
The production of prochymosin directed by a cloned cDNA under the control of a trp promoter was examined in E. coli C600 and HB101. The latter host exhibited a higher degree of expression as to the production of prochymosin in the form of inclusion bodies, which accounted for more than 15-20% of the total cellular protein. The conditions for the processing of prochymosin in the inclusion bodies to active chymosin were determined. Several enzymatic properties of the processed bacterial chymosin, such as its specific activities as to milk-clotting and proteolysis, heat stability and Ca2+ dependence of the clotting activity, were almost identical to those of authentic chymosin. However, a slight difference was observed with regard to the immunological reactivity with anti-prochymosin antibody.
- 社団法人 日本農芸化学会の論文
著者
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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KAWAGUCHI Yoshiyuki
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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KOSUGI Shigeo
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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SASAKI Katsutoshi
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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