Cleavage of Potato Virus Y Polyprotein in Escherichia coli Depending on the Proteolytic Activity of Viral Protease
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概要
- 論文の詳細を見る
We have cloned a 5-kb cDNA corresponding to the 3'-half of genomic RNA of potato virus Y (PVY), the type member of plant potyvirus. Based on its nucleotide sequence, the cDNA was presumed to encode PVY protease responsible for the self-processing of polyprotein precursor expressed from PVY genome. To test its proteolytic activity, the cDNA was modified so as to express the protease-polymerase-coat protein (CP) portion of PVY polyprotein in Escherichia coli. By Western blotting analysis of the cell extract of E. coli expressing the polyprotein, mature CP was detected. A large deletion in the polymerase-coding region did not affect the emergence of mature CP, but a small deletion in the putative protease region resulted in disappearance of mature CP and appearance of the intact polyprotein. These results indicated that the polyprotein expressed in E. coli was cleaved depending on the proteolytic activity of PVY protease.
- 社団法人日本農芸化学会の論文
- 1993-05-23
著者
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HIDAKA Makoto
Department of Biotechnology, The University of Tokyo
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MASAKI Haruhiko
Department of Biotechnology, The University of Tokyo
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Hidaka Makoto
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Uozumi T
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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YAMAOKA Yukiho
Government Industrial Research Institute
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UOZUMI Takeshi
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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Masaki Haruhiko
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Yoshida Y
Government Industrial Research Institute
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Uozumi Takeshi
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Yoshida Yukiko
Department Of Chemical Pharmacology Toho University School Of Pharmaceutical Sciences
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Masaki H
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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