Isolation of the creA Gene from the Cellulolytic Fungus Humicola grisea and Analysis of CreA Binding Sites Upstream from the Cellulase Genes
スポンサーリンク
概要
- 論文の詳細を見る
A carbon catabolite repressor gene, creA, was isolated from the cellulolytic fungus Humicola grisea by using a portion of the Trichoderma reesei cre1 gene as a probe. The deduced amino acid sequence predicts a zinc finger protein of 419 amino acids in length, and its zinc finger regions show high similarity with those of Aspergillus CreAs, T. reesei Cre1,and Saccharomyces cerevisiae MIG1. Northern blot analysis showed that the H. grisea creA gene was highly transcribed when the mycelia were grown on glucose-containing media, but the transcription of the H. grisea endoglucanase 1 gene (egl1) and the exoglucanase 1 gene (exo1) were repressed under these conditions. Results of binding assays with the maltose-binding protein : : CreA (1-166) fusion protein and the egl1 and the exo1 upstream regions showed that some 6-bp sites having an identical or similar sequence to the consensus sequence for CreA binding were protected from DNase I digestion.
- 社団法人日本農芸化学会の論文
- 1998-12-23
著者
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HIDAKA Makoto
Department of Biotechnology, The University of Tokyo
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MASAKI Haruhiko
Department of Biotechnology, The University of Tokyo
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Hidaka Makoto
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Nakamura A
Fuji Oil Co. Ltd. Jpn
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Nakamura Akira
Department ofRespirology, Graduate School of Medicine, Chiba University
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Uozumi T
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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UOZUMI Takeshi
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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Masaki Haruhiko
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Nakamura Akira
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Uozumi Takeshi
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Masaki H
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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TAKASHIMA Shou
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of To
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Nakamura A
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Nakamura Akira
Department Oc Macromolecular Science Faculty Of Science Osaka University
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