Cloning, Sequencing, and Expression of a Thermostable Cellulase Gene of Humicola grisea
スポンサーリンク
概要
- 論文の詳細を見る
The egl2 gene encoding a thermostable endoglucanase (EGL2) was cloned from Humicola grisea. The DNA sequence of egl2 predicted two putative introns in the coding region. The deduced amino acid sequence of EGL2 was 388 amino acids in length and showed 99.5% identity with the H. insolens CMC 3. In addition to TATA box and CAAT motifs, putative CREA binding sites were observed in the 5' upstream region of the egl2 gene. The egl2 gene was expressed in Aspergillus oryzae, and EGL2 was purified. EGL2 produced by A. oryzae showed a high activity toward carboxymethyl cellulose. The optimal temperature of EGL2 was 75℃, and EGL2 had more than 80% residual activity after heating up to 75℃ for 10 min. This is the first report of enzymatic properties of the EGL2-type thermostable cellulase homologs from Humicola.
- 社団法人日本農芸化学会の論文
- 1997-02-23
著者
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MASAKI Haruhiko
Department of Biotechnology, The University of Tokyo
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Nakamura Akira
Department ofRespirology, Graduate School of Medicine, Chiba University
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Uozumi T
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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UOZUMI Takeshi
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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Masaki Haruhiko
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Nakamura Akira
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Uozumi Takeshi
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Masaki H
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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TAKASHIMA Shou
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of To
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Nakamura A
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Nakamura Akira
Department Oc Macromolecular Science Faculty Of Science Osaka University
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