Oxygen Sensitivity of NifA Protein of Azospirillum lipoferum FS as Suggested by Gene Cloning and Expression in Escherichia coli
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概要
- 論文の詳細を見る
We cloned and sequenced a 2.8-kb SalI fragment of Azospirillum lipoferum FS as a homologue of the Klebsiella oxytoca nifA gene. The amino acid sequence deduced from an open reading frame of 1872 bases showed 91% identity to that of the A. brasilense NifA, and the putative central σ^<54> interaction domain was conserved as well as the C-terminal DNA-binding domain. The NifA function on the nifH promoter was examined in Escherichia coli using a combination of a nifA driver plasmid and a nifH-lacZ reporter plasmid, in which the transcriptional activation of the nifH promoter by the NifA was evaluated with the β-galactosidase activity. The A. lipoferum NifA activated the nifH promoter solely under microaerobic conditions, while the K oxytoca NifA activated it irrespective of the oxygen condition. These observations suggest that oxygen sensitivity is an intrinsic property of the A. lipoferum NifA.
- 社団法人日本農芸化学会の論文
- 1997-05-23
著者
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HIDAKA Makoto
Department of Biotechnology, The University of Tokyo
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MASAKI Haruhiko
Department of Biotechnology, The University of Tokyo
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Hidaka Makoto
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Shigematsu Toru
Department of Materials and Life Science, Graduate School of Science and Technology, Kumamoto Univer
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INOUE Akio
Department of Orthopedics, Kurume University School of Medicine
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Uozumi T
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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UOZUMI Takeshi
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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Shigematsu Toru
Department Of Applied Chemistry And Biochemistry Faculty Of Engineering Kumamoto University
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Shigematsu Toru
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Masaki Haruhiko
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Uozumi Takeshi
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Masaki H
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Inoue Akio
Department Of Biological Sciences Graduate School Of Science Osaka University
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Inoue Akio
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Inoue Akio
Department Of Astronomy Faculty Of Science Kyoto University
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