Influence of Ni^<2+> and Co^<2+> on Methanogenic Activity and the Amounts of Coenzymes Involved in Methanogenesis

概要

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The requirement of Ni^<2+> and Co^<2+> addition on methanogenic activity and the coenzymes involved in methanogenesis were investigated in anaerobic continuous cultivation with synthetic wastewater using acetate as the sole carbon source. Addition of Ni^<2+> and Co^<2+> to the synthetic wastewater drastically increased the maximum dilution rate of the cultivation. The concentrations of coenzymes F_<430> and corrinoids in the biomass increased to 0.62 μmol-Ni/g-VSS and 0.67 μmol-Co/g-VSS, respectively with the increase of the dilution rate. Methanogenic activity of the culture broth also increased with an increase of dilution rate. However, without addition of Ni^<2+> and Co^<2+> F_<430> and corrinoids were not detected in the biomass and methanogenic activity was only a trance level at a dilution rate of 0.025 d^<-1>. When the amounts of Ni^<2+> and Co^<2+> added at a dilution rate of 0.6 d^<-1> were lowered in steps, the concentrations of F_<430> and corrinoids in the biomass and methanogenic activity decreased with decreasing amounts of Ni^<2+> and Co^<2+> added. These results suggest that Ni^<2+> and Co^<2+> were required for the methane-producing reactions via increases of coenzymes F_<430> and corrinoids.
社団法人日本生物工学会の論文
2001-06-25