Production of Protease Using Wastewater from the Manufacture of Shochu

概要

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The production of protease using wastewater from a shochu distillery was investigated in order to devise a process for the treatment of shochu distillery wastewater. Aspergillus usami mut. shirousami IFO 6082 was selected from among eight strains for production of protease. Production of 240 U/ml of proteolytic activity was achieved after 72 h in a jar-fermentor culture under the following conditions : initial pH, 5; 30℃; aeration, 1 vvm; and agitation, 600 rpm. The protease was purified by column chromatography on Sephadex G-150 and isoelectrofocusing. The molecular weight of the purified enzyme determined by SDS-PAGE was 56 kDa, and the isoelectric point was pH 4.0. The optimum pH for the reaction was about 4.0,so the enzyme is therefore an acid protease. The optimum temperature for proteolysis ranged between 55 and 60℃, while the enzyme was unstable at temperatures above 60℃.
社団法人日本生物工学会の論文
1994-02-25