Repeated-Batch Ethanol Fermentation by a Flocculating Yeast, Saccharomyces cerevisiae IR-2

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Repeated-batch fermentation by a flocculating yeast, Saccharomyces cerevisiae IR-2,was set up in a molasses medium using an automatically controlled system, and the effect of temperature and mash density were studied. At 30℃, a concentration of ethanol of 106g/l with a productivity of 2.5g/l・h, which was 2.5 time higher than that of the fed-batch fermentation process used commercially in Japan, and a fermentation efficiency of 83% were obtained in a molasses medium that initially contained 25%(w/v)sugar. However, raising the temperature from 30℃ to 33℃ and then to 35℃ had a very detrimental effect on S. cerevisiae IR-2,even in a molasses medium with 20%(w/v)sugar. The specific growth rate(μ)and the specific production rate of ethanol(q)could by represented in terms of ethanol concentration as follows : μ=μ_<max>(1-P/P_g), and q=q_<max>(1-P/P_e), where P represents the concentration of ethanol. Increasing the initial concentration of sugar had little effect on the values of P_g(the concentration of ethanol above which yeast cells do not grow)and P_e(the concentration of ethanol above which yeast cells do not produce ethanol), which were about 78 and 104g/l, respectively. Raising the temperature caused a decrease in P_g and P_e, which fell to 32 and 65g/l at 35℃, respectively. The repeated-batch fermentation process using S. cerevisiae IR-2 gave good results at 30℃, but it did not proceed well at higher temperatures.
社団法人日本生物工学会の論文
1991-05-25

Repeated-Batch Ethanol Fermentation by a Flocculating Yeast, Saccharomyces cerevisiae IR-2

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