Ethanol Production by Repeated-Batch Fermentation at High Temperature in a Molasses Medium Containing a High Concentration of Total Sugar by a Thermotolerant Flocculating Yeast with Improved Salt-Tolerance

概要

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An attempt was made to improve the salt tolerance of the thermotolerant flocculating yeast Saccharomyces cerevisiae strain KF-7 (KF-7) by maintaining a high concentration of KCl in the medium. Among selected strains, K211 had the highest cell viability and ethanol productivity in a molasses medium containing 25% (w/v)total sugar at 35℃. Strain K211 accumulated higher concentrations of glycogen and trehalose than did KF-7,and also remained alive in the stationary phase during batch fermentation in a jar fermentor. As a result of repeated-batch fermentation tests with K211,stable ethanol production was achieved with an ethanol concentration of 92 g/l and a productivity of 3.5 g/l・h at 33℃ in 22% molasses medium. With KF-7,stable ethanol production could not be attained under these conditions. In addition, even at the higher temperature of 35℃, strain K211 gave stable ethanol production with an ethanol concentration of 91 g/l and a productivity of 2.7 g/l・h.
社団法人日本生物工学会の論文
1997-03-25