Intraspecific Hybridization by Protoplast Fusion in Mucorales Producing Milk-clotting Proteases(Microbiology & Fermentation Industry)
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概要
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The properties of the intraspecific hybrids obtained by protoplast fusion of (+) and (-) mating type strains of Mucor pusillus producing the milk-clotting protease were studied, especially as to their mating types and enzyme productivities. All of the prototrophic hybrid strains obtained from the (+)ade/hyp+(-)cho fusion and three adenine-requiring segregants obtained on serial subcultivation formed zygospores autonomously. In the case of the (-) cho +(-) met fusion, most of the prototrophic hybrids had the (-) mating type, however, two of the 25 non-sectored hybrids were found to be homothallic and formed zygospores autonomously. Although the enzyme productivities of the intraspecific hybrids were lower than that of Mucor miehei, which is closely related to Mucor pusillus, the hybrids showed distinctly higher productivity than the parental Mucor pusillus strains.
- 社団法人日本農芸化学会の論文
- 1986-06-23
著者
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Goto-hamamoto Makiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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BEPPU Teruhiko
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok
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UOZUMI Takeshi
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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OHNUKI Tetsuo
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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Ohnuki Tetsuo
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Uozumi T
Univ. Tokyo Tokyo Jpn
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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