Characterization of Nitrile Hydratase Genes Cloned by DNA Screeing from Rhodococcus erythropolis
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概要
- 論文の詳細を見る
Southern hybridization analysis using the genes encoding the α- and β-subunits of nitrile hydratase (NHase) from Rhodococcus sp. N-774 as probe suggested that two R. erythropolis strains, JCM6823 and JCM2892, among 31 strains mainly from Japan Culture of Microorganisms (JCM) have NHase genes. Restriction analysis of DNA fragments showing positive hybridization showed that each fragment carried a nucleotide sequence very similar to that of the NHase genes from Rhodococcus sp. N-774. Nucleotide sequence analysis of the DNA fragment cloned from R. erythropolis JCM6823 showed the presence of the genes encoding the α- and β-subunits of NHase, which show 94.7% and 96.2% identity in amino acid sequence to those of Rhodococcus sp. N-774, respectively, as well as a C-terminal portion of the amidase gene upstream from these genes. Despite the extremely high amino acid sequence similarity in both NHases and amidases from R. erythropolis JCM6823 and Rhodococcus sp. N-774, the NHases and amidases from R. erythropolis strains showed broader substrate specificity when compared to those from Rhodococcus sp. N-774. This suggests that a very limited number of amino acid residues are responsible for the difference in substrate specificity. Although the NHase of Rhodococcus sp. N-774 are constitutively produced, the NHases of both R. erythropolis strains were inducibly produced by addition of ε-caprolactam as an inducer.
- 社団法人日本農芸化学会の論文
- 1993-08-23
著者
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HORINOUCHI Sueharu
Department of Biotechnology, University of Tokyo
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BEPPU Teruhiko
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok
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Nishiyama M
Biotechnology Research Center The University Of Tokyo
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Nishiyama Makoto
Biotechnology Research Center The University Of Tokyo
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Nishiyama Makoto
Department Of Orthopaedic Surgery Tokyo Medical University
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Nishiyama Makoto
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Horinouchi Sueharu
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Horinouchi Sueharu
Dep. Of Biotechnology Graduate School Of Agriculture And Life Sciences Univ. Of Tokyo
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Beppu T
Department Of Applied Biological Science Nihon University
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Duran Robert
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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Duran Robert
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nishiyama Makoto
Atopy (Allergy) Research Center, Juntendo University School of Medicine
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