Suppression of an Ethanol-Sensitive Mutation of Acetobacter pasteurianus by Overexpression of the his1 Gene Encoding Histidinol Phosphate Aminotransferase
スポンサーリンク
概要
- 論文の詳細を見る
An ethanol-sensitive mutant strain of Acetobacter pasteurianus was isolated by chemical mutagenesis with N-methyl-N'-nitrosoguanidine. The his1 gene of A. pasteurianus, which codes for histidinol phosphate aminotransferase (HPAT), was cloned on a multicopy plasmid pMV24 because of its ability of complement the ethanol-sensitive mutation. The mutant was found still to possess almost the same HPAT activity as the parental strain, which was increased to five times that the basal activity by the introduction of a multicopy plasmid containing the his1 gene into the mutant. It was thus conculded that an increase in the HPAT activity led to the suppression of the ethanol-sensitive mutation.
- 社団法人日本生物工学会の論文
- 1993-09-25
著者
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HORINOUCHI Sueharu
Department of Biotechnology, University of Tokyo
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BEPPU Teruhiko
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok
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Takemura Hiroshi
(present Address)bio-polymer Research Co. Ltd.
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Takemura Hiroshi
Bio-polymer Research Co. Ltd.:(present Address)nakano Central Institute Nakano Vinegar Co. Ltd.
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Horinouchi Sueharu
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Horinouchi Sueharu
Dep. Of Biotechnology Graduate School Of Agriculture And Life Sciences Univ. Of Tokyo
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Beppu T
Department Of Applied Biological Science Nihon University
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Tagami Haruko
Nakano Central Research Institute Nakano Vinegar Co. Ltd.
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Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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