A Calcium-binding Protein with Four EF-hand Motifs in Streptomyces ambofaciens
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概要
- 論文の詳細を見る
A gene (cabA) encoding a calcium-binding protein was cloned from Streptomyces ambofaciens. CabA was 180 amino acid residues long and contained four typical EF-hand motifs bearing high sequence similarity to the calcium-binding sites in calmodulin. Consistent with this, CabA showed distinct calcium-binding activity, comparable to bovine brain calmodulin. cabA was transcribed throughout growth, as found by S1 nuclease mapping. Southern hybridization experiments showed that a single copy of cabA was present in various Streptomyces species. A hypothetical relationship between CabA and aerial mycelium formation in this strain was examined, since S. ambofaciens showed calcium-dependent aerial mycelium formation. However, disruption of cabA or overexpression of cabA in S. ambofaciens caused no detectable phenotypic changes.
- 社団法人日本農芸化学会の論文
- 2001-01-23
著者
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Horinouchi Sueharu
Department Of Biotechnology Graduate School Of Agriculture And Life Sciences The University Of Tokyo
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Horinouchi Sueharu
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Ohnishi Yasuo
Department Of Biotechnology Graduate School Of Agriculture And Life Sciences The University Of Tokyo
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Ohnishi Yasuo
Department Of Biotechnolog Graduate School Of Agriculture And Life Sciences The University Of Tokyo
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YONEKAWA Tohru
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok
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Yonekawa Tohru
Department Of Biotechnology Graduate School Of Agriculture And Life Sciences The University Of Tokyo
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