Identification of the Cellulose-Binding Domain of a Bacillus subtilis Endoglucanase Distinct from Its Catalytic Domain
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概要
- 論文の詳細を見る
The endoglucanase (BSC) from Bacillus subtilis IFO 3034, which shows no ability to hydrolyze microcrystalline cellulose, was found to bind to Avicel. Ninety-eight amino acids-truncation at the COOH-terminus of BSC did not abolish the carboxymethyl cellulose (CMC)-hydrolyzing ability, but removed the Avicel-binding ability. These data suggested the presence of an Avicel-binding domain at the COOH-terminus of BSC, despite its inability to hydrolyze crystalline cellulose. A mutant enzyme with Phe at the 131st His, generated by site-directed mutagenesis, had no enzymatic activity with CMC as the substrate, as predicted from hydrophobic cluster analysis, while the cellulose-binding ability of the mutant enzyme still remained. Similarly, the mutation at the 169th Glu severely affected the enzyme activity, but not the cellulose-binding ability. All these data clearly show that BSC is composed of the catalytic domain at its NH_2-terminal portion and the cellulose-binding domain at its COOH-terminal portion, and that the two domains are independently functional in the absence of the other.
- 社団法人日本農芸化学会の論文
- 1993-02-23
著者
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HORINOUCHI Sueharu
Department of Biotechnology, University of Tokyo
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Nakamura Akira
Department ofRespirology, Graduate School of Medicine, Chiba University
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BEPPU Teruhiko
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok
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Horinouchi Sueharu
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Horinouchi Sueharu
Dep. Of Biotechnology Graduate School Of Agriculture And Life Sciences Univ. Of Tokyo
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Nakamura Akira
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Park J‐s
Dr. Chung's Food Co. Ltd. Choongchungbuk‐do Kor
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Beppu T
Department Of Applied Biological Science Nihon University
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Park Jae-Seon
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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Nakamura A
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nakamura Akira
Department Oc Macromolecular Science Faculty Of Science Osaka University
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