Isolation of a Genetic Marker Linked to the Bh Gene by Genetically Directed Representational Difference Analysis of Closed Colony Japanese Quails(Genetics)

スポンサーリンク

概要

• 論文の詳細を見る

• Disappearance of the coloration pattern of the plumage and whole body hemorrhage is caused by an autosomal dominant black at hatch (Bh) mutation in the Japanese quail. In this study, we adopted genetically directed representational difference analysis (GDRDA) to obtain a genetic marker linked to Bh from our closed colony stock. A DNA pool of 10 wild-type (+/+) quails was subtracted from another DNA pool of 10 quails heterozygous for the Bh mutation (Bh/+). After analysis of 106 clones isolated by three series of GDRDA using three different restriction enzymes, one clone was shown to detect a restriction fragment length polymorphism (RFLP) between +/+ and Bh/+ quails. A genomic region flanking the polymorphic restriction site was cloned by inverse-PCR, and a PCR-RFLP maker linked to the Bh mutation was developed. The marker showed no recombination with the Bh mutation in 101 quails analyzed. This is the first report in which a genetic marker linked to a specific gene was successfully isolated by GDRDA using a closed colony population, and this strategy is expected to have wide applications in species whose genetic resources are not well established.

• 社団法人日本動物学会の論文
• 2001-01-20

著者

• Ushijima Toshikazu

  Carcinogenesis Division, National Cancer Center Research Institute

• Nakamura A

  Fuji Oil Co. Ltd. Jpn

• Nakamura Akira

  Department ofRespirology, Graduate School of Medicine, Chiba University

• YAMASHITA Satoshi

  Carcinogenesis Division, National Cancer Center Research Institute

• SHIOJIRI NOBUYOSHI

  Department of Biology, Faculty of Science, Shizuoka University

• Nakamura Akira

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Niwa Tohru

  Department of Biology, Faculty of Science, Shizuoka University

• Ushijima Toshikazu

  Carcinogenesis Div. National Cancer Center Res. Inst.

• Ushijima T

  Carcinogenesis Division National Cancer Center Research Institute

• Niwa Tohru

  Graduate School Of Science And Technology Shizuoka University

• Ushijima Toshikazu

  国立がんセンター研究所 発がん研究部

• Nakamura A

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Yamashita Satoshi

  Carcinogenesis Division National Cancer Center Research Institute

• Shiojiri N

  Shizuoka Univ. Shizuoka Jpn

• Shiojiri Nobuyoshi

  Department Of Biology Faculty Of Science Shizuoka University

• Nakamura Akira

  Department Oc Macromolecular Science Faculty Of Science Osaka University

関連論文

• Increased H-ras mutation frequency in mammary tumors of rats initiated with N-methyl-N-nitrosourea (MNU) and treated with acrylamide
• Lack of association between CpG island methylator phenotype in human gastric cancers and methylation in their background non-cancerous gastric mucosae
• Frequent hypomethylation in multiple promoter CpG islands is associated with global hypomethylation, but not with frequent promoter hypermethylation
• β-Catenin mutations and nuclear accumulation during progression of rat stomach adenocarcinomas
• Cloning of the 5' Upstream Region of the Rat p16 Gene and Its Role in Silencing
• Cloning of the 5' Upstream Region of the Rat p16 Gene and Its Role in Silencing
• Linkage Mapping of the Bra, Brb and Brg Genes for Rat Protein Phosphatase 2A 55 kDa B-Regulatory Subunit Isotypes
• Decreased Lipoprotein Lipase in Obstructive Sleep Apnea Syndrome
• Magnetization Measurements and ^Rb-NMR on Rb_2M_3S_4(M=Ni and Pd) with the Kagome Lattice
• Bilateral Breast Cancer in a Patient with a Strong Family History of Cancer; Analysis of p53 Germ Line Mutations
• Chemical genomic screening for methylation-silenced genes in gastric cancer cell lines using 5-aza-2'-deoxycytidine treatment and oligonucleotide microarray
• Difference in Target Organs in Carcinogenesis with a Heterocyclic Amine, 2-Amino-3,4-dimethylimidazo[4,5-f]quinoline, in Different Strains of Mice
• Linkage Mapping of the Rat Msh2 DNA Mismatch Repair Gene on Chromosome 6
• Demonstration of ras and p.53 Gene Mutations in Carcinomas in the Forestomach and Intestine and Soft Tissue Sarcomas Induced by N-Methyl-N-nitrosourea in the Rat
• Identification of 29 Rat Genetic Markers by Arbitrarily Primed Polymerase Chain Reaction
• Rare Activation of the Human c-Ha-ras Transgene of Mice in Hemangioendothelial Sarcomas and Liver Tumors Induced by Glu-P-1
• EFFECT OF TEA CATECHINS ON GASTRIC SUBMUCOSAL CIRCULATION
• Synthesis and Properties of a Nitridomolybdenum(VI) Complex Having Benzenedithiolate Ligands. Effect of the Thiolate on the Activation of the Nitrido Ligand
• Thermal Conductivity of BaCo_Ni_xS_2
• Gene Expression Profiling Distinguishes Between Spontaneous and Radiation-induced Rat Mammary Carcinomas
• Isolation of Flavohemoglobin from the Actinomycete Streptomyces antibioticus Grown without External Nitric Oxide Stress
• Unusual Transcription Regulation of the niaD Gene under Anaerobic Conditions Supporting Fungal Ammonia Fermentation
• Denitrification of Nitrate by the Fungus Cylindrocarpon tonkinense(Biochemistry & Molecular Biology)
• A Possible Role of NADPH-Dependent Cytochrome P450nor Isozyme in Glycolysis under Denitrifying Conditions(Biochemistry & Molecular Biology)
• Formate-forming Fungal Catabolic Pathway to Supply Electrons to Nitrate Respiration(Biochemistry & Molecular Biology)
• Decision Problem for a Logic of Temporal Information
• Variation in Conductivity of Cadmium Sulfo-Selenide Sintered Films with Vacuum Heat Treatment
• Methylation-associated silencing of the Wnt antagonist SFRP1 gene in human ovarian cancers
• Coupling of Tearing Modes in Normal RFP Discharge
• Observation of m=0 Structure with Streak Picture in a Reversed Field Pinch Having Large Magnetic Reynolds Number
• The Absence of Mth1 Inactivation and DNA Polymerase κ Overexpression in Rat Mammary Carcinomas with Frequent A:T to C:G Transversions
• Linkage Mapping of the Rat Interleukin 1 Receptor Antagonist Gene on Chromosome 3
• Differentiation of Biliary Epithelial Cells from the Mouse Hepatic Endodermal Cells Cultured in Vitro
• STUDIES ON MOLYBDO-OXIDASE MODELS : AIR OXIDATION OF PPH_3 BY Mo (VI) COMPLEXES OF CYSTEINE-CONTAINING PEPTIDES
• Dioxomolybdenum(VI) and Dioxotungsten(VI) Structure with Mono(Dithiolato) Coordination: Crystal Structures, Properties and Reactivity of [{M^O_2(dithiolato}_2(μ-O)]^ (M=Mo and W) Complexes
• Molecular Assembly Micellization of Molybdenum(V,IV) Thiolate and Selenolate Complexes with Long Hydrocarbon Chains
• Synthesis and Structures of(Porphinato)(thiolato)gallium(III)Complexes
• Stabilization of [4Fe-4S] Ferredoxin Model Complex by a Combination of Hydrophobic Cholyl Group and the Specific NH・・・S Hydrogen Bond in Aqueous Micellar Solution
• Cooperative Redox Regulation of[4Fe-4S] Ferredoxin Model Arenethiolate Complexes by NH【triple bond】S Hydrogen Bonds and an Aromatic C-H【triple bond】S Interaction
• Unique Axial Interaction of a Quadruply-Bonded Cr(II)-Cr(II) with Two Pt(II) Atomas in the Linearly Aligned Pt-Cr-Cr-Pt Supported by Four 6-Diphenylphosphino-2-pyridonate Ligands
• Theoretical Studies of Multiple Metal-Metal Bonds between Divalent Molybdenum Ions in Diners, Tetramers, and Clusters
• Combined Effect of Bulkiness and NH・・・S Hydrogen Bonding Controls the Formation of Terminal and Bridging Hydrazine Ruthenium(II) Complexes with Thiolate Ligands
• Large Third-Order Nonlinear Optical Susceptibilities of Multiply-Bonded M_2(pyphos)_4 and M_2Pd_2Cl_2(pyphos)_4(M=Cr, Mo ; Pyphos=6-Diphenylphosphino-2-pyridonate) by Picosecond Degenerate Four-Wave Mixing Method
• Benzoin Oxidation at Extreme Temperature by Bis(1, 2-benzenedithiolato)dioxotungstate(VI)Complex : A Model Study for Hyperthermostable Tungsten Oxidoreductases
• Stabilization of Oxo-Metal Bonding by the π-Conjugated System in Dithiolate Ligands : cis-Dioxotungsten(VI) Bis(naphthalenedithiolato) and the Related Complexes as Models for Tungsten Oxidoreductases
• Selective Synthesis of Tungsten(VI)Mono(catecholato)Complexes Using 1, 2-Bis(trimethylsiloxy)benzenes : Molecular Structure of WCl_4(O_2C_6H_4)
• A Comparison between the Structures of Tungstopterin Cofactor in Tungsten Oxidoreductase and a Model Complex, cis-Dioxobis (2,3-naphthalenedithiolate) tungsten (VI) in Their O-Atom Transfer Reaction
• Japanese Late-Onset Breast Cancer Families: Their Clinicopathological Characteristics and Absence of BRCA1 and BRCA2 Germline Mutations
• BRCA1 and BRCA2 Germline Mutations in Japanese with Hereditary Breast Cancer Families
• Anaerobic Elemental Sulfur Reduction by Fungus Fusarium oxysporum
• Isolation of a Genetic Marker Linked to the Bh Gene by Genetically Directed Representational Difference Analysis of Closed Colony Japanese Quails(Genetics)
• The Stablization of Dioxomolybdenum(VI) Dithiolene Complex by the Electron Withdrawing Substituent on the Dithiolene Ligand: Comparison of Reactivities of Monooxomolybdenum(IV) Complex with R_2S_2C_2(R= -CN and -COOMe) Ligands as Models of Molybdenum Oxid
• Promoter methylation profiling of 30 genes in human malignant melanoma
• Disappearance of serum HCV-RNA after short-term prednisolone therapy in a patient with chronic hepatitis C associated with autoimmune hepatitis-like serological manifestations
• Generation of a Polymorphic Marker Linked to Thymoma Susceptibility Gene of Rat 1 by Genetically-Directed Representational Difference Analysis
• Aberrant DNA methylation in contrast with mutations
• Experiments on Flow-Induced In-Line Oscillation of a Circular Cylinder in a Water Tunnel : 2nd Report, Influence of the Aspect Ratio of a Cantilevered Circular Cylinder
• Experiments on Flow-Induced In-Line Oscillation of a Circular Cylinder in a Water Tunnel : 1st-Report, The Difference of the Response Characteristics When a Cylinder is Elastically Supported at Both Ends and Cantilevered
• Identification of the Cellulose-Binding Domain of a Bacillus subtilis Endoglucanase Distinct from Its Catalytic Domain
• Persistence of a component of DNA methylation in gastric mucosae after Helicobacter pylori eradication
• DNA methylation : a marker for carcinogen exposure and cancer risk
• DNA methylation as a marker for the past and future
• Cloning of a New cryIA(a) Gene from Bacillus thuringiensis Strain FU-2-7 and Analysis of Chimaeric CryIA(a) Proteins for Toxicity
• Ectopically expressed PIR-B on T cells constitutively binds to MHC class I and attenuates T helper type 1 responses
• Enzymatic Analysis of a Thermostabilized Mutant of an Escherichia coli Hygromycin B Phosphotransferase
• ISOLATION OF RFLP MARKERS LINKED TO Bh GENE BY GDRDA-WEEC OF JAPANESE QUAILS (COTURNIX JAPONICA)(Genetics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• EXPRESSION OF GENES RELATING TO a-MSH SIGNALING IN THE PIGMENT PATTERN FORMATION OF FEATHER BUDS IN QUAIL EMBRYOS(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• Transcriptional Regulation of the Bacillus ohbensis Cyclodextrin Glucanotransferase Gene in B. subtilis
• Isolation and Characterization of the Actin Gene from the Cellulolytic Fungus Humicola grisea and Analysis of Transcription Levels of Actin and Cellulase Genes
• Simple Experiments of DCOM, JAVA IDL and RMI
• Simple Experiments of DCOM,JAVA IDL and RMI (Javaシステム)
• Molecilar Clpmomg amd Expression of the Novel Fungal β-Glucsidase Gennes from Humicola grisea and Trichoderma reesei^1
• Isolation of the creA Gene from the Cellulolytic Fungus Humicola grisea and Analysis of CreA Binding Sites Upstream from the Cellulase Genes
• Novel Mast Cell Lines with Enhanced Proliferative and Degranulative Abilities Established from Temperature-Sensitive SV40 Large T Antigen Transgenic Mice
• Isolation of the Gene and Characterization of the Enzymatic Properties of a Major Exoglucanase of Humicola grisea without a Cellulose-Binding Domain
• Cloning of a Gene Encoding a Putative Xylanase with a Cellulose-Binding Domain from Humicola grisea
• Cloning, Sequencing, and Expression of a Thermostable Cellulase Gene of Humicola grisea
• Enhanced Expression of cryIA(a) Gene of Bacillus thuringiensis in Escherichia coli
• Cloning of a Gene Encoding a Putative Carbon Catabolite Repressor from Trichodermareesei
• Purification and Characterization of Cellulases from Humicola grisea
• Extracellular Production of Bacillus ohbensis Cyclodextrin Glucanotransferase by B. subtilis
• Use of a Triple Protease-deficient Mutant of Bacillus subtilis as a Host for Secretion of a B. subtilis Cellulase and TEM β-Lactamase(Microbiology & Fermentation Industry)
• Mapping of Vanadium-Related Luminescence on SiC Wafer at Room Temperature
• Anaerobic Elemental Sulfur Reduction by Fungus Fusarium oxysporum
• Effects of Oral Supplementation with Cystine and Theanine on the Immune Function of Athletes in Endurance Exercise : Randomized, Double-Blind, Placebo-Controlled Trial
• Synthesis, Structure and Catalytic Activity of a Seven - Coordinate Titanium (IV) Tris(pyridine - 2 - thiolate) Complex
• Plasma insulin and glucose levels in elderly female subjects with Alzheimer's disease
• Lanthanoid Complexes of Azobenzene and Benzo[c]cinnoline Derived from Metallic Lanthanoids : Crystal Structures of Binuclear [SmI(thf)_3(μ-η^2 : η^2-trans-PhNNNPh)SmI(thf)_3] and Mononuclear [Yb(benzo[c]cinnoline)_3(thf)_2]
• IMMUNOLOCALIZATION OF HEPATOCYTE GROWTH FACTOR (HGF) AND ITS RECEPTOR DURING MOUSE LIVER REGENERATION(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• ANALYSIS OF FETAL HEPATOBLAST-NONPARENCHYMAL CELL INTERACTIONS BY USING PRIMARY CELL CULTURE SYSTEM(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• Construction of a Bacillus subtilis Strain Deficient in Three Proteases(Biological Chemistry)
• Both Humoral Mesenchymal Factors and the Close Association between the Hepatic Endoderm and Mesenchyme can be Involved in Liver Formation of Mouse Embryos(Developmental Biology)
• Establishment and Functional Characterization of Novel Natural Killer Cell Lines Derived from a Temperature-Sensitive SV40 Large T Antigen Transgenic Mouse
• Rational Preparation and Characterization of Tandem Tetrametal Clusters. Crystal Structure of Tetrakis (6-diphenylphosphino-2-pyridonato) dimolybdenum
• Human Chorionic TSH (hCTSH, hCT) during Normal or Molar Pregnancy
• Reconstruction of the Small Intestine from the Dissociated Cells
• Preparation and Structural Characterization of Cationic Dinuclear Ruthenium(II) -Thiolate Complexes, [Ru_2(SPh)_3(η^6-p-cymene)_2]Y (Y = Cl and PF_6)
• 16-Electron Half-sandwich Ru(II)-Thiolate Complexes, Ru(SAr)_2(η^6-p-cymene) (Ar = 2,6-dimethylphnyl and 2,4,6-triisopropylphenyl)
• LOCALIZATION OF THE TIMING SYSTEM FOR THE ONSET OF GASTRULATION IN XENOPUS EMBRYOS(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• PURIFICATION OF MOUSE FETAL HEPATOBLASTS AND THEIR PRIMARY CULTURE(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)

もっと見る 閉じる

スポンサーリンク