Gene Expression Profiling Distinguishes Between Spontaneous and Radiation-induced Rat Mammary Carcinomas
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概要
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The ability to distinguish between spontaneous and radiation-induced cancers in humans is expected to improve the resolution of estimated risk from low dose radiation. Mammary carcinomas were obtained from Sprague-Dawley rats that were either untreated (n = 45) or acutely γ-irradiated (1 Gy; n = 20) at seven weeks of age. Gene expression profiles of three spontaneous and four radiation-induced carcinomas, as well as those of normal mammary glands, were analyzed by microarrays. Differential expression of identified genes of interest was then verified by quantitative polymerase chain reaction (qPCR). Cluster analysis of global gene expression suggested that spontaneous carcinomas were distinguished from a heterogeneous population of radiation-induced carcinomas, though most gene expressions were common. We identified 50 genes that had different expression levels between spontaneous and radiogenic carcinomas. We then selected 18 genes for confirmation of the microarray data by qPCR analysis and obtained the following results: high expression of Plg, Pgr and Wnt4 was characteristic to all spontaneous carcinomas; Tnfsf11, Fgf10, Agtr1a, S100A9 and Pou3f3 showed high expression in a subset of radiation-induced carcinomas; and increased Gp2, Areg and Igf2 expression, as well as decreased expression of Ca3 and non-coding RNA Mg1, were common to all carcinomas. Thus, gene expression analysis distinguished between spontaneous and radiogenic carcinomas, suggesting possible differences in their carcinogenic mechanism.
- 日本放射線影響学会の論文
- 2008-07-16
著者
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Ushijima Toshikazu
Carcinogenesis Division, National Cancer Center Research Institute
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Kakinuma Shizuko
放射線医学総合研究所放射線防護研究センター 発達期被ばく影響研究グループ
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Kakinuma Shizuko
Experimental Radiobiology For Children's Health Research Group Research Center For Radiation Pr
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Shimada Yoshiya
Experimental Radiobiology For Children's Health Research Group Research Center For Radiation Pr
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Kakinuma Shizuko
放射線医学総合研究所 発達期被ばく影響研究グループ
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IMAOKA Tatsuhiko
Experimental Radiobiology for Children's Health Research Group, Research Center for Radiation Protec
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YAMASHITA Satoshi
Carcinogenesis Division, National Cancer Center Research Institute
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NISHIMURA Mayumi
Experimental Radiobiology for Children's Health Research Group, Research Center for Radiation Protec
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SHIMADA Yoshiya
National Institute of Radiological Sciences
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Imaoka Tatsuhiko
Experimental Radiobiology For Children's Health Research Group Research Center For Radiation Pr
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Imaoka Tatsuhiko
Department Of Biological Sciences Graduate School Of Science University Of Tokyo:department Of Molec
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Shang Yi
Experimental Radiobiology For Children's Health Research Group National Institute Of Radiologic
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Shimada Yoshiya
Experimental Radiobiology For Children's Health Res. Group Res. Center For Radiation Protection
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Ushijima Toshikazu
Carcinogenesis Div. National Cancer Center Res. Inst.
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Ushijima Toshikazu
国立がんセンター研究所 発がん研究部
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Nishimura Mayumi
Experimental Radiobiology For Children's Health Research Group Research Center For Radiation Pr
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Nishimura Mayumi
Low Dose Radiat. Eff. Res. Proj. Nirs
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Shimada Yoshiya
Experimental Radiobiology For Children's Health Research Group Research Center For Radiation Pr
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Yamashita Satoshi
Carcinogenesis Division National Cancer Center Research Institute
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Nishimura Mayumi
Low-dose Radiation Effect Project Radiation Safety Research Center Nirs
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SHIMADA Yoshiya
Experimental Biology for Children's Health Research Group, National Institute of Radiological Sciences
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NISHIMURA Mayumi
Experimental Biology for Children's Health Research Group, National Institute of Radiological Sciences
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KAKINUMA Shizuko
Experimental Biology for Children's Health Research Group, National Institute of Radiological Sciences
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