Transcriptional Regulation of the Bacillus ohbensis Cyclodextrin Glucanotransferase Gene in B. subtilis
スポンサーリンク
概要
- 論文の詳細を見る
The expression of the cyclodextrin glucanotransferase (CGTase) gene (cgt) of Bacillus ohbensis, when introduced into an α-amylase-defective strain of B. subtilis on a multicopy plasmid, pHY300PLK, was induced in the presence of starch and was subject to catabolite repression by glucose as well as in the original strain, B. ohbensis. We constructed a cgt' : : 'lacZ translational fusion to study the expression in B. subtilis, and this construct was confirmed to be subject to both starch induction and catabolite repression. In order to define the region involved in the regulation of the cgt gene, a series of cgt' : : 'lacZ gene with various lengths of deletion in the promoter region was constructed on pHY300PLK. DNA regions responsible for starch induction and catabolite repression by glucose could be separated in the deletion experiment. Primer extension analysis showed that the catabolite repression was controlled at the initiation of transcription, while the starch induction is likely to be controlled by a transcriptional termination-antitermination mechanism.
- 社団法人日本農芸化学会の論文
- 1999-11-23
著者
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MASAKI Haruhiko
Department of Biotechnology, The University of Tokyo
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Nakamura A
Fuji Oil Co. Ltd. Jpn
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Nakamura Akira
Department ofRespirology, Graduate School of Medicine, Chiba University
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Uozumi T
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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UOZUMI Takeshi
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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Masaki Haruhiko
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Nakamura Akira
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Uozumi Takeshi
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Masaki H
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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NISHIDA Tamotsu
Department of Biotechnology, The University of Tokyo
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Nakamura A
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Nishida Tamotsu
Department Of Biotechnology The University Of Tokyo:(present Address) School Of Life Science Tokyo U
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Nakamura Akira
Department Oc Macromolecular Science Faculty Of Science Osaka University
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