Cloning of a New cryIA(a) Gene from Bacillus thuringiensis Strain FU-2-7 and Analysis of Chimaeric CryIA(a) Proteins for Toxicity
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概要
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We cloned the cryIA(a) gene from Bacillus thuringiensis strain FU-2-7, one of the toxin genes encoding lepidopteran-specific protoxins. Sequence analysis of the gene showed two amino acid differences (Pro77 to Leu and Phe965 to Ser) from the CryIA(a) of B. thuringiensis strain HD-1. We constructed chimaeric cryIA(a) genes using FU-2-7 and HD-1 cryIA(a) genes and isolated the chimaeric protoxins, as well as the parental ones, from Escherichia coli cells harboring the recombinant plasmids to examine the effects of the two amino acid variations on the toxicity. FU-2-7 CryIA(a) protein was about half as toxic against the smaller tea tortrix, Adoxophyes sp. , and one-third as toxic against the silkworm, Bombyx mori, as that of HD-1. On the other hand, a chimaeric CryIA(a) protein with a single replacement of Phe965 to Ser had nearly the same toxicity as the HD-1 CryIA(a) against the smaller tea tortrix and one-third the toxicity against silkworm as that of HD-1. This improved property of the chimaeric CryIA(a) protoxin may be useful for widening its application to crop protection in sericultural countries.
- 社団法人日本農芸化学会の論文
- 1994-05-23
著者
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MASAKI Haruhiko
Department of Biotechnology, The University of Tokyo
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Mori H
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Mori Hironori
Department Of Food Science And Technogloy Faculty Of Agriculture
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Mori Hironori
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Nakamura Akira
Department ofRespirology, Graduate School of Medicine, Chiba University
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Uozumi T
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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UOZUMI Takeshi
Department of Biotechnology, Faculty of Agriculture, The University of Tokyo
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Masaki Haruhiko
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Nakamura Akira
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Uozumi Takeshi
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Masaki H
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Udayasuriyan Varatharajalu
Bioscience Department, Tsukuba Research Laboratory, Toagosei Chemical Industry Company Ltd.
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Nakamura A
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Udayasuriyan Varatharajalu
(present Address)centre For Plant Molecular Biology Tamil Nadu Agricultural University
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Nakamura Akira
Department Oc Macromolecular Science Faculty Of Science Osaka University
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