Cloning and Expression of the Momordica charantia Trypsin Inhibitor II Gene in Silkworm by using a Baculovirus Vector
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概要
- 論文の詳細を見る
MCTI-II (Momordica charantia trypsin inhibitor II) isolated from bitter gourd (Momordica charantia LINN.) seeds is one of the serine protease inhibitors of the squash family. We cloned cDNA that encodes MCTI-II and constructed an expression system for MCTI-II by using a baculovirus vector. The recombinant baculovirus was inoculated to early fifth-instar larvae of the silkworm (strain : Shunrei×Shougetsu). Four days after infection, the hemolymph of silkworm larvae was collected and the recombinant protein was purified. Two kinds of expressed MCTI-II protein were obtained. An amino acid sequence analysis of the two proteins indicates that both were similar to the authentic inhibitor, except for the addition of a tripeptide derived from the vector at the N-terminus. One of the two inhibitors (MCTI-II A) resulted in a single PTH-amino acid in each Edman degradation cycle, while the other (MCTI-II B) resulted in two PTH-amino acids, suggesting the occurrence of cleavage of the reactive site. The inhibitory activities of MCTI-II expressed toward trypsin are examined in terms of the K_i value, these being 6.4×10^<-10>M for MCTI-II A and 5.2×10^<-10>M for MCTI-II B.
- 社団法人日本農芸化学会の論文
- 2000-02-23
著者
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Mori H
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Mori Hironori
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Kamei K
Kyoto Inst. Technol. Kyoto Jpn
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SATO SHINICHI
Departments of Internal Medicine, Sakura National Hospital
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Mori Hajime
Department of Applied Biology, Kyoto Institute of Technology
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SATO Hideki
Department of Reparative Materials, Field of Tissue Engineering, Institute for Frontier Medical Scie
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KAMEI Kaeko
Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute
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HARA Saburo
Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute
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TAKANO Ryo
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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Sato Shinichi
From The Department Of Applied Biology Kyoto Institute Of Technology
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Ichida Masatoshi
Experimental Farms Faculty Of Textile Science Kyoto Institute Of Technology
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Ishihara M
National Defense Medical College Research Institute
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Taniguchi Masatoshi
Department Of Applied Biology Kyoto Institute Of Technology
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TANIGUCHI Mai
Department of Applied Biology, Kyoto Institute of Technology
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Takano Ryo
Department Of Applied Biology Kyoto Institute Of Technology
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Hara Saburo
Energy & Mechanical Engineering Department Central Research Institute Of Electric Power Industry
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Hara Saburo
Department Of Chemistry And Materials Technology Faculty Of Engineering And Design
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Mori Hajime
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Kudo Ken-ichi
Sanwa Cornstarch Co. Ltd
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Sato H
Hokkaido Univ. Sapporo Jpn
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Hara Saburo
Department Of Applied Biology Kyoto Institute Of Technology
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Kamei Kaeko
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Sato Shinichi
Department Of Dermatology Faculty Of Medicine University Of Tokyo
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Sato Shin-ichi
Department Of Chemistry And Materials Technology Faculty Of Engineering And Design Kyoto Institute O
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Sato Hideki
Department Of Applied Physics Graduate School Of Engineering Tohoku University
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Sato Shinichi
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Sato Hideki
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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Sato Shin-ichi
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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Kamei Kaeko
Department fo Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology
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