Expression of YAV Proteins and Vaccination against Viral Ascites among Cultured Juvenile Yellowtail
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概要
- 論文の詳細を見る
Yellowtail ascites virus (YAV) is a member of the family Birnaviridae and causes viral ascites among juvenile yellowtail (Seriola quinqueradiata). We have reported the cloning and expression of two viral cDNAs, the first being segment A encoding a polyprotein of viral capsid proteins (VP2 and VP3) and a protease (NS), and the second being VP2-epitope encoding serotype-specific epitope region on VP2,using a baculovirus expression system. Another viral cDNA encoding a polyprotein of NS and VP3 was cloned and expressed in this study. For the expression of NS/VP3 (YAV nt 1626 to 3066) in insect cells a 31-kDa protein, corresponding to VP3 was detected, indicating an appropriate posttranslational processing of NS/VP3 polypeptide by NS protease itself. The analysis of the N-terminal amino acid sequence of this protein showed that NS protease may cleave an Ala-Ser bond. A study of the potential for vaccination of yellowtail fry by injection of insect cell lysates infected with baculovirus, containing either cDNA of segment A, VP2-epitope, or NS/VP3 was undertaken. Only a vaccination with celll lysates infected with a recombinant virus carrying the full length of YAV segment A gene demonstrated approximately the same effect as that of inactivated YAV. This result suggested that all proteins VP2,VP3,and NS are required for an effective vaccination.
- 社団法人日本農芸化学会の論文
- 2000-07-23
著者
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Mori H
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Mori Hironori
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Kamei K
Kyoto Inst. Technol. Kyoto Jpn
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Maeno Yukio
National Research Institute Of Aquaculture
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Maeno Yukio
National Research Institute Of Aquaculture Fisheries Agency
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Mori Hajime
Department of Applied Biology, Kyoto Institute of Technology
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Nakajima K
National Research Institute Of Aquaculture Fisheries Agency
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KAMEI Kaeko
Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute
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HARA Saburo
Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute
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TAKANO Ryo
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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KUDO Ken-ichi
Sanwa Cornstarch Co., Ltd.
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Takano Ryo
Department Of Applied Biology Kyoto Institute Of Technology
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Hara Saburo
Energy & Mechanical Engineering Department Central Research Institute Of Electric Power Industry
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Hara Saburo
Department Of Chemistry And Materials Technology Faculty Of Engineering And Design
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Mori Hajime
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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SATO Hiedki
Department of Applied Biology, Kyoto Institute of Technology
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NAKAJIMA Kazuhiro
National Research Institute of Aquaculture, Fisheries Agency
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KAMAISHI Takashi
National Research Institute of Aquaculture, Fisheries Agency
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KAMATA Tatsuo
Department of Applied Biology, Kyoto Institute of Technology
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Kudo Ken-ichi
Sanwa Cornstarch Co. Ltd
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Sato H
Hokkaido Univ. Sapporo Jpn
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Kamata Tatsuo
Department Of Applied Biology Kyoto Institute Of Technology
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Kamaishi Takashi
Department Of Medical Biology School Of Medicine Showa University
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Kamaishi Takashi
National Research Institute Of Aquaculture Fisheries Agency
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Hara Saburo
Department Of Applied Biology Kyoto Institute Of Technology
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Kamei Kaeko
Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Nakajima Kazuhiro
National Research Institute Of Aquaculture Fisheries Agency
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Sato Hideki
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology
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Kamei Kaeko
Department fo Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology
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