Reusability of Broth Supernatant as Medium

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Reusability of seupernatants of culture broths as media was examined in fed-batch culture of Protaminobacter ruber, Candida brassicae and Escherichia coli.After each microorganism was cultivated up to a high cell concentration (60-130 g/l) with a DO-stat, the broth supernatant was used again to make up the medium for the next cultivation. In the cases of P. ruber and C. brassicae, growth rates were almost the same on repeated use, four times, of the supernatant.In the case of E. coli, however, the bacterium could not grow on the medium prepared with the supernatant. This might be due to accumulation of acetic acid, one of the metabolic products, to an inhibitory lovel for the growth. It was possible to use the broth supernatant of E. coli as a medium for cultivation of C. brassicae. A simple procedure for preparaton of the medium using the supernatant was proposed, in which amounts of the components to be supplemented to the medium were calculated as products of the cell mass and the predetermied consumption rates.
社団法人日本生物工学会の論文
1980-06-25