Construction of a Novel Hydroxyproline-Producing Recombinant Escherichia coli by Introducing a Proline 4-Hydroxylase Gene
スポンサーリンク
概要
- 論文の詳細を見る
An Escherichia coli recombinant strain producing trans-4-hydroxy-L-proline(Hyp) was constructed by introducing a proline 4-hydroxylase gene into an L-proline-producing E.coli. Plasmid pOF1, which contains a gene encoding feedback resistant γ-glutamyl kinase(proB74), was constructed and introduced into E.coli W1485 putA. The recombinant E.coli W1485 putA/pPF1 strain produced L-proline(1.2 g/l). The proline production by W1485 putA/pPF1 was converted to Hyp production by introducing pWFH1 which contains a proline 4-hydroxylase gene. E.coli W1485 putA which harbors pWFP1 carrying the proline 4-hydroxylase gene, proB74, and proA produced 25 g/l of Hyp in 96 h. A novel biosynthetic pathway of Hyp, which has not previously been produced in E.coli, was constructed in E.coli.
- 社団法人日本生物工学会の論文
- 2000-11-25
著者
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Mori H
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Mori Hironori
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Ozaki A
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Ozaki Akio
Technical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Ozaki Akio
Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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SHIBASAKI TAKESHI
Development Department, Bio-chemicals Company, Kyowa Hakko Kogyo Co.Ltd.
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HASHIMOTO SHINICHI
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.
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MORI HIDEO
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co.Ltd.
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Shibasaki Takeshi
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.: (present Address) Technical Research Laborat
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Hashimoto Shinichi
Project Group Of Risk Assessment And Management For Endocrine Disruptor And Dioxin-like Compounds Na
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