Cloning, Purification, and Properties of a Cofactor-independent Glutamate Racemase from Lactobacillus brevis ATCC 8287
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概要
- 論文の詳細を見る
A glutamate racemase gene of Lactobacillus brevis ATCC 8287 was cloned into Escherichia coli TM93 by the phenotypic complementation of a phosphoenolpyruvate carboxylase deficiency on minimum agar medium containing D-glutamate. The gene was localized to a 1.4-kb HindIII-EcoRI DNA fragment and the total nucleotide sequence of the fragment was analyzed. The gene has typical promoter and SD sequences which appeared to function in E. coli. The deduced amino acid sequence of the enzyme had 276 amino acids and the molecular weight was calculated as 29, 426. Two cysteine residues and their surrounding regions of the enzyme are homologous to those of other cofactor-independent racemases. The glutamate racemase was puritied from recombinant E. coli to homogeneity and characterized. The enzyme required no cofactors for the activity, and retained its activity even in 2M (300g/l) L-glutamate.
- 社団法人日本農芸化学会の論文
- 1995-04-23
著者
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Yagasaki Makoto
Technical Research Laboratories, Kyowa Hakko Bio Co., Ltd.
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Azuma M
Osaka City Univ. Osaka Jpn
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Yagasaki M
Kyowa Hakko Kogyo Co. Ltd. Yamaguchi Jpn
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Yagasaki Makoto
Technical Research Laboratories Kyowa Hakko Bio Co. Ltd.
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Yagasaki Makoto
Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Azuma Masaki
Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Ozaki A
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Ozaki Akio
Technical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Ozaki Akio
Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Iwata Kazuhisa
Technical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Ishino Shuichi
Technical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Iwata Kazuhisa
Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Ishino S
Kyowa Hakko Kogyo Co. Ltd. Yamaguchi Jpn
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Ishino Shuichi
Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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