Expression and Characterization of Sucrose Synthase from Mung Bean Seedlings in Escherichia coli
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概要
- 論文の詳細を見る
The cDNA fragment coding for mung bean (Vigna radiata Wilczek) sucrose synthase was introduced into the expression vector pET-20b resulting in the construction of plasmid pEB-01. After transformation of Escherichia coli strain BL21(DE3) cells by pEB-01 and induction with isopropyl thio-β-galactoside, high level expression of the recombinant enzyme was obtained. The enzyme had a tetrameric form that conserved the activity of sucrose synthase. Although the K_m and V_<max> of the recombinant enzyme acting on either UDP-glucose or fructose were very close to those of the native enzyme isolated from mung bean seedlings, the K_m for sucrose was higher by a factor of 10 for the recombinant enzyme. This suggests that the recombinant sucrose synthase has a tendency to synthesize sucrose, although the native enzyme catalyzes a freely reversible reaction.
- 社団法人日本農芸化学会の論文
- 1997-09-23
著者
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Tahara N
Wood Research Institute Kyoto University
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Nakai Tomonori
Wood Research Inst. Kyoto Univ.
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Mori H
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Tonouchi N
Ajinomoto. Co. Inc. Kawasaki Jpn
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Mori Hironori
Department Of Biotechnology Faculty Of Agriculture The University Of Tokyo
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Sakai Fukumi
Wood Research Institute, Kyoto University
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Hayashi Takahisa
Wood Research Institute, Kyoto University
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Sakai F
Kyoto Univ.
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Tonouchi Naoto
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Tonouchi Naoto
Bio-polymer Research Co. Ltd.
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Tonouchi Naoto
Bio-polymer Research Co. Ltd. Ksp R&d Business-park Bldg.
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Tsuchida Takayasu
Bio-Polymer Research Co.Ltd., KSP
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Mori Hitoshi
Faculty of Agriculture, Nagoya University
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Hayashi Tomoyuki
Laboratory Of Engineered Wood And Joints Department Of Wood Engineering Forestry And Forest Products
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Hayashi Tomoyuki
Engineered Timber And Joints Laboratory Forestry And Forest Products Research Institute
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Hayashi T
Wood Research Institute Kyoto University
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Sakai Fukumi
Wood Research Institute Kyoto University
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Hayashi Takahisa
Wood Res. Inst. Kyoto Univ.
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Hayashi T
Laboratory Of Engineered Wood And Joints Department Of Wood Engineering Forestry And Forest Products
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Tsuchida T
Bio-polymer Research Co. Ltd.
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Tsuchida Takayasu
Central Research Laboratories Of Ajinomoto Co. Inc.
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Tsuchida Takayasu
Bio-polymer Research Co. Ltd.
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Tsuchida T
Bio-polymer Res. Co. Ltd.
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HAYASHI Takahisa
Wood Res. Inst. , Kyoto University, Gokasho, Uji, Kyoto
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