Construction of a Vector Plasmid for Use in Gluconobacter oxydans(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
A host vector system in Gluconobacter oxydans was constructed. An Acetobacter-Escherichia coli shuttle vector was introduced with the efficiency of 10^4 transformants/μg of DNA. Next, aiming for a self-cloning vector, we found a cryptic plasmid (which we named pAG5) of 5648 bp in G. oxydans strain IFO 3171, and sequenced the nucleotides. The plasmid seemed to have only one open reading flame (ORF) for a possible replication protein. Shuttle vectors of Gluconobacter-E. coli were constructed with the plasmid pAG5 and an E. coli vector, pUC18.
- 社団法人日本農芸化学会の論文
- 2003-01-23
著者
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Yokozeki Kenzo
AminoScience Laboratories, Ajinomoto Co., Inc.
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Tonouchi N
Ajinomoto. Co. Inc. Kawasaki Jpn
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Yokozeki K
Ajinomoto. Co. Inc. Kawasaki Jpn
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Yokozeki Kenzo
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Yokozeki Kenzo
Aminoscience Laboratories Ajinomoto Co. Inc.
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TONOUCHI Naoto
AminoScience Laboratories, Ajinomoto Co., Inc.
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SUGIYAMA Masakazu
AminoScience Laboratories, Ajinomoto Co., Inc.
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Tonouchi Naoto
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Tonouchi Naoto
Bio-polymer Research Co. Ltd. Ksp R&d Business-park Bldg.
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Sugiyama Minoru
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Sugiyama M
Aminoscience Laboratories Ajinomoto. Co. Inc.
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