Structural Characterization of the 16-kDa Allergen, RA17,in Rice Seeds. Prediction of the Secondary Structure and Identification of Intramolecular Disulfide Bridges
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概要
- 論文の詳細を見る
The 16-kDa rice allergen, RA17,belonging to the α-amylase/trypsin imhibitor family was isolated from rice seed and structurally characterized by identifying cystine-containing peptides and predicting the secondary structure and hydrophobic regions. Eight peptides, which constitute three sets of cystine-containing peptides, were purified by HPLC from a thermolytic digest of RA17 and identified by their amino acid sequence and composition, Indicating five intramolecular disulfide bridges : Cyd^<34>-Cys^<94>, Cys^<26>-(Cys^<50> or Cys^<51>)-Cys^<110> and Cys^<12>-(Cys^<62> or Cys^<64>)-Cys^<122>. Analyses of the CD spectrum and the Chou-Fasman prediction suggested that RA17 had some helical-and sheet-structure regions. Based on these experimental and predicted data, RA17 is proposed to be a globular molecule with a small hydrophobic core having folding restricted by five intramolecular disulfide bridges.
- 社団法人日本農芸化学会の論文
- 1999-12-23
著者
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MATSUDA Tsukasa
Department of Food Science ande Tecknology, School of Agriculture, Nagoya University
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NAKAMURA Ryo
Department of Food Science ande Tecknology, School of Agriculture, Nagoya University
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Nakamura R
Nihon Univ. Setagaya Jpn
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Nakamura R
Meijo Univ. Nagoya Jpn
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Nakamura Ryo
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Matsuda T
Department Of Applied Molecular Biosciences School Of Bioagricultural Sciences Nagoya University
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Matsuda T
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Matsuda Tsukasa
School Of Agriculture Nagoya University
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Matsuda Tsukasa
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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IZUMI Hidehiko
Department of Food and Nutrition, Nagoya College of Nutrition
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SUGIYAMA Minoru
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University
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Izumi Hidehiko
Department Of Food And Nutrition Nagoya College Of Nutrition
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Sugiyama Minoru
Aminoscience Laboratories Ajinomoto. Co. Inc.
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Matsuda Tsukasa
Department Of Applied Biological Science School Of Agricultural Science Nagoya University
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Sugiyama Minoru
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Nakamura Ryo
Department Of Applied Biological Sciences School Of Agricultural Science Nagoya University
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MATSUDA Tsukasa
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University
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IZUMI Hidehiko
Department of Food and Nutrition, Nagoya College of Nutrition:Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University
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NAKAMURA Ryo
Department of Food Science and Technology, Nihon University
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