The Bovine Lactophorin C-Terminal Fragment and PAS6/7 Were Both Potent in the Inhibition of Human Rotavirus Replication in Cultured Epithelial Cells and the Prevention of Experimental Gastroenteritis
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概要
- 論文の詳細を見る
Rotaviruses are the leading cause of severe dehydrating diarrhea in children worldwide. We have found that high-Mr glycoprotein fraction (F1) from cow’s milk whey has potent inhibitory activity against human rotavirus (HRV) in cell culture. The present study was undertaken to identify and characterize the components responsible for this inhibitory activity. F1 was initially heated at 95 °C for 30 min, rendering milk antibodies inert, subjected to ammonium sulfate fractionation, and then resolved by two-dimensional polyacrylamide gel electrophoresis. After electroelution, we found that a heat-stable milk protein lactophorin C-terminal fragment (LP16) and bovine milk fat globule membrane protein PAS6/7 strongly inhibited the replication of HRV MO strains in MA104 cells. Furthermore, we found that prophylactic oral administration of F1 once before inoculation of the HRV MO strain obviously prevented the development of diarrhea in vivo. These non-immunoglobulin components are a promising candidate for a prophylactic food additive against HRV infection.
- 社団法人 日本農芸化学会の論文
- 2010-07-23
著者
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Nagaoka Satoshi
Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University
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KANAMARU Yoshihiro
Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University
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NAKAGOMI Osamu
Department of Microbiology, Akita University School of Medicine
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MATSUDA Tsukasa
Department of Food Science ande Tecknology, School of Agriculture, Nagoya University
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Nagai Sayaka
Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University
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Nakagomi Osamu
Department Of Microbiology Akita University School Of Medicine
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Minamoto N
Veterinary Medicine Fac. Of Applied Biological Sciences Gifu Univ.
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Minamoto Nobuyuki
The United Graduate School Of Veterinary Sciences Gifu University
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Minamoto Nobuyuki
Department Of Veterinary Public Health Faculty Of Agriculture Gifu University
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Matsuda Tsukasa
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Minamoto Nobuyuki
Veterinary Medicine Fac. Of Applied Biological Sciences Gifu Univ.
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金丸 美敬
Dep. Of Applied Life Sci. Gifu Univ.
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Kanamaru Yoshihiro
Department Of Food Science Faculty Of Agriculture Gifu University
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Kanamaru Yoshihiro
Department Of Food Science Faculty Of Applied Biological Science Gifu University
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Ito Naoto
Gifu Univ. Gifu Jpn
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NAKAGOMI Toyoko
Department of Microbiology, Akita University School of Medicine
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Nagai Sayaka
Department Of Applied Life Science Gifu University
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Kanamaru Yoshihiro
Department Of Applied Life Science Faculty Of Applied Biological Sciences Gifu University
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Kanamaru Yoshihiro
Faculty Of Applied Biological Sciences Gifu University
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INAGAKI Mizuho
United Graduate School of Agricultural Science, Gifu University
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YABE Tomio
Department of Applied Life Science, Gifu University
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TAKAHASHI Takeshi
Food Science Institute, Division of Research and Development, Meiji Dairies Co., Ltd.
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EBINA Takusaburo
Sendai Institute of Microbiology
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Nakagomi Osamu
Department Of Molecular Microbiology And Immunology Graduate School Of Biomedical Sciences And Globa
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Nagaoka Satoshi
Department Of Applied Life Science Faculty Of Applied Biological Sciences Gifu University
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Nagaoka Satoshi
Department Of Applied Life Science Gifu University
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Matsuda Tsukasa
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Matsuda Tsukasa
Department Of Applied Biological Science School Of Agricultural Science Nagoya University
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Inagaki Mizuho
Department Of Applied Life Science Gifu University
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Yabe Tomio
Department Of Applied Life Science Gifu University
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Nakagomi Toyoko
Department Of Molecular Microbiology And Immunology Graduate School Of Biomedical Sciences And Globa
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Nakagomi Toyoko
Department Of Microbiology Akita University School Of Medicine
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Takahashi Takeshi
Food Science Institute Division Of Research And Development Meiji Dairies Co. Ltd.
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Inagaki Mizuho
Department of Applied Life Science, Gifu University
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Yabe Tomio
United Graduate School of Agricultural Science, Gifu University
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NAGAOKA Satoshi
Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University
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