Suppressive expression of the glutelin A1-collagen peptide fusion gene in hybrid rice lines with the mutant line, Low Glutelin Content-1 (LGC-1)
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概要
- 論文の詳細を見る
A low glutelin-content mutant, LGC-1, has a mutant gene (lgc1) comprising a tail-to-tail inverted repeat of two highly homologous glutelin B genes (gluB4 and δ-gluB5), resulting in the production of double-stranded glutelin B RNA. Hybrid rice lines between LGC-1 and a transgenic line with the glutelin A1-collagen peptide fusion gene (gluA1-coll) were produced, and F2 hybrid lines homozygous for each or both of the gluA1-coll and lgc1 genes were selected. The expression of gluA1-coll in F2 lines with lgc1 was far lower than that of F2 lines without lgc1 at both the mRNA and protein levels as measured by semi-quantitative RT-PCR and immunoblotting using anti-collagen peptide antibody. The gluA1 mRNA sequence alignment identical to the potential double-stranded RNA formed by gluB4 sense and δ-gluB5 antisense RNAs consisted of 14 nucleotides at most, and was not more than 23 even when one mismatch was allowed within the sequence. Thus, such shorter double-stranded glutelin B RNAs were suggested to suppress the expression of not only endogenous gluA genes as reported previously (Kusaba et al. 2003) but also the exogenous gluA1-coll in the hybrid lines.
- 日本植物細胞分子生物学会の論文
- 2008-12-01
著者
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Aoki Naohito
Department of Life Sciences, Faculty of Bioresources, Mie University
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Okajima Tetsuya
Dep. Of Biochemistry Ii Graduate School Of Medicine Nagoya Univ.
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日野 真吾
名古屋大学大学院生命農学研究科
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MATSUDA Tsukasa
Department of Food Science ande Tecknology, School of Agriculture, Nagoya University
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Aoki Naohito
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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NADANO Daita
Department of Legal Medicine, Fukui Medical School
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Matsuda Tsukasa
Dep. Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Univ.
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HASHIZUME Fujio
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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HINO Shingo
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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OKAJIMA Tetsuya
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Matsuda Tsukasa
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Hino Shingo
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Nadano Daita
Department Of Legal Medicine Fukui Medical School
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Matsuda Tsukasa
Department Of Applied Biological Science School Of Agricultural Science Nagoya University
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Nadano Daita
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Hashizume Fujio
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Okajima Tetsuya
Department Of Anatomy Nagoya University School Of Medicine
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Hino Shingo
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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