Immunochemical and Biochemical Identification of the Rice Seed Protein Encoded by cDNA Clone A3-12
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概要
- 論文の詳細を見る
Previously isolated cDNA clone A3-12 that was expressed in E. coli as the fusion protein with Trp E showed immunoreactivity with the mouse antibody raised against isolated α-globulin from rice seed. The N-terminal amino acid sequences determined for the purified α-globulin and its tryptic peptides were identical with the deduced amino acid sequence reported, except for two residues at the protein N terminus. An error in the reported sequence was confirmed by re-sequencing the cDNA, the nucleotide sequence for the two N-terminal residues being shown to be CA<GC>^^^TG and not CA<CG>^^^TG. Thus, the protein encoded by cDNA clone A3-12 was identified to be the major rice seed globulin, α-globulin, with an apparent molecular mass of 26 kDa.
- 社団法人日本農芸化学会の論文
- 1996-06-23
著者
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Adachi Takahiro
Department of immunology, Medical Research Institute, Tokyo Medical and Dental University
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Aoki Naohito
Department of Life Sciences, Faculty of Bioresources, Mie University
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Adachi Takahiro
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Alvarez A
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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MATSUDA Tsukasa
Department of Food Science ande Tecknology, School of Agriculture, Nagoya University
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NAKAMURA Ryo
Department of Food Science ande Tecknology, School of Agriculture, Nagoya University
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Aoki N
Yamaguchi Univ. Yamaguchi Jpn
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Aoki Naohito
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Adachi T
Med. Res. Inst. Tokyo Med. Dent. Uni.
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Nakamura R
Nihon Univ. Setagaya Jpn
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Nakamura R
Meijo Univ. Nagoya Jpn
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Nakamura Ryo
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Matsuda T
Department Of Applied Molecular Biosciences School Of Bioagricultural Sciences Nagoya University
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Matsuda T
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Matsuda Tsukasa
School Of Agriculture Nagoya University
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NAKASE Masayuki
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University
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ALVAREZ Angelina
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University
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NAKASE Masayuki
Grad. Sch. of Bioagr. Sci., Nagoya Uni.
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Matsuda Tsukasa
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Alvarez Angelina
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Matsuda Tsukasa
Department Of Applied Biological Science School Of Agricultural Science Nagoya University
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Nakase Masayuki
Grad. Sch. Of Bioagr. Sci. Nagoya Uni.
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Nakamura Ryo
Department Of Applied Biological Sciences School Of Agricultural Science Nagoya University
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