Isolation and Culture of Bovine Mammary Epithelial Cells and Establishment of Gene Transfection Conditions in the Cells
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概要
- 論文の詳細を見る
Bovine mammary epithelial cells (BMEC) were isolated as acinous fragments from a mammary gland of a lactating cow. They grew well on plastic substratum, showed the characteristic cobblestone morphology of epithelial cells, and secreted α_<s1>-, β-, and k-caseins even when grown on plastic substratum. A plasmid containing the bacterial chloramphenicol acetyl transferase (CAT) gene was transfected to the isolated BMEC by calcium phosphate precipitation and electroporation methods. The transfection efficiency of BMEC by the calcium phosphate method was greatly improved by post-transfection osmotic shock with glycerol or polyethylene glycol. An about 700 bp DNA fragment containing 5'-flanking sequence of bovine α_<s1>-casein gene showed promoter activity in the transfected BMEC. The primary culture of BMEC might be useful for studies on regulation of bovine milk-protein gene expression.
- 社団法人日本農芸化学会の論文
- 1995-01-23
著者
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Adachi Takahiro
Department of immunology, Medical Research Institute, Tokyo Medical and Dental University
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Aoki Naohito
Department of Life Sciences, Faculty of Bioresources, Mie University
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Ahn Jung-youb
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Adachi Takahiro
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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MATSUDA Tsukasa
Department of Food Science ande Tecknology, School of Agriculture, Nagoya University
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NAKAMURA Ryo
Department of Food Science ande Tecknology, School of Agriculture, Nagoya University
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Aoki N
Yamaguchi Univ. Yamaguchi Jpn
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Aoki Naohito
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Adachi T
Med. Res. Inst. Tokyo Med. Dent. Uni.
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Nakamura R
Nihon Univ. Setagaya Jpn
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Nakamura R
Meijo Univ. Nagoya Jpn
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Nakamura Ryo
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Matsuda T
Department Of Applied Molecular Biosciences School Of Bioagricultural Sciences Nagoya University
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Matsuda T
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Matsuda Tsukasa
School Of Agriculture Nagoya University
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Mizuno Yuri
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University
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Matsuda Tsukasa
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Mizuno Yuri
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Matsuda Tsukasa
Department Of Applied Biological Science School Of Agricultural Science Nagoya University
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Nakamura Ryo
Department Of Applied Biological Sciences School Of Agricultural Science Nagoya University
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