Absorption, Migration and Kinetics in Peripheral Blood of Orally Administered Ovalbumin in a Mouse Model
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概要
- 論文の詳細を見る
Intestinal absorption of food proteins is well known, whereas its physiological significance remains to be investigated. Various amounts (1, 10 and 50 mg) of ovalbumin were orally administered to mice and the blood kinetics were subsequently analyzed by two-site ELISA. The blood ovalbumin concentration consistently reached its maximum (7–90 ng/ml) about 20 min after the oral administration and then gradually decreased in a dose-dependent manner. Only intact (45 kDa) and truncated (40 kDa) ovalbumins were always detected in the blood independently of the administration site, intra-stomach or intra-intestine, while various fragments of the protein were observed in the gastrointestinal lumen after the oral administration. Recognition by a specific monoclonal antibody and an acidic shift of its pI value suggested that the 40-kDa truncated ovalbumin was produced by intracellular limited proteolysis at its C-terminus. Such stable absorption and blood kinetics of undigested ovalbumin in normal mice suggest some sort of physiological significance for the intestinal uptake of intact food proteins.
- 社団法人 日本農芸化学会の論文
- 2008-10-23
著者
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Aoki Naohito
Department of Life Sciences, Faculty of Bioresources, Mie University
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HONJOH Tsutomu
Morinaga Institute of Biological Science, Inc.
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Honjoh Tsutomu
Morinaga Institute Of Biological Science Inc.
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MATSUDA Tsukasa
Department of Food Science ande Tecknology, School of Agriculture, Nagoya University
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Aoki Naohito
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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NADANO Daita
Department of Legal Medicine, Fukui Medical School
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Matsuda Tsukasa
Dep. Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Univ.
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OKAJIMA Tetsuya
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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MIZUMACHI Koko
National Institute of Livestock and Grassland Science
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Matsuda Tsukasa
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Nadano Daita
Department Of Legal Medicine Fukui Medical School
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Mizumachi Koko
National Institute Of Animal Industry
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Matsubara Takeshi
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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KURISAKI Jun-ichi
Jumonji University
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Kurisaki Jun-ichi
Jumonji Univ.
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Matsuda Tsukasa
Department Of Applied Biological Science School Of Agricultural Science Nagoya University
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Nadano Daita
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Matsubara Takeshi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Okajima Tetsuya
Department Of Anatomy Nagoya University School Of Medicine
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HONJOH Tsutomu
Morinaga Institute of Biological Science
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