Sandwich Enzyme-Linked Immunosorbent Assay of Canine Leptin.
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概要
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Leptin is the ob gene product secreted from adipocytes in mammals, and thereby its plasma level reflects body fat content. To establish an assay method for leptin in the dog, rabbit anti-canine leptin antibody was obtained using canine recombinant leptin as an antigen. This antibody reacted to canine leptin much stronger than mouse, rat and human leptins. Sandwich enzyme-linked immunosorbent assay (ELISA) using this antibody was developed. The serum leptin levels of 13 healthy dogs were in a range from 1.4 to 5.6 ng/ml with the mean ± SEM of 3.0 ± 0.3 ng/ml.
- 公益社団法人 日本獣医学会の論文
著者
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Honjoh Tsutomu
Morinaga Institute Of Biological Science Inc.
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Sasaki Noriyasu
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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Kimura Kazuhiro
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Kyoto University
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Komagome Rika
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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SAITO Masayuki
Department of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University
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KIMURA Kazuhiro
Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
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IWASE Miho
Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
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ISHIOKA Katumi
Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
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HONJOH Tsutomu
Morinaga Institute of Biological Science
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HONJOH Tsutomu
Morinaga Institute of Biological Science, Tsurumi-ku, Yokohama 230-8504, Japan
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